S100b-RAGE-mediated augmentation of angiotensin II-induced activation of JAK2 in vascular smooth muscle cells is dependent on PLD2

Sean S. Shaw, Ann Marie Schmidt, Amy K. Banes, Xiaodan Wang, David Stern, Mario B. Marrero

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Angiotensin II (Ang II), a vasoactive peptide that is also considered a growth factor, has been implicated in both normal and diabetic cellular proliferation. We recently found that activation of janus kinase 2 (JAK2) is essential for the Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) and that high glucose augments Ang II-induced proliferation of VSMCs by increasing signal transduction through activation of JAK2. Here, we demonstrate that S100B, a ligand for the receptor of advanced glycation end products (RAGEs), augmented both Ang II-induced tyrosine phosphorylation of JAK2 and cell proliferation in VSMCs in a receptor-dependent manner. We also found that S100B-RAGE interaction triggered intracellular generation of reactive oxygen species (ROS), VSMC proliferation, and JAK2 tyrosine phosphorylation via activation of phospholipase D (PLD)2. These results provide direct evidence for linkages between PLD2, ROS production, and S100B-RAGE-induced enhancement of Ang II-induced cell proliferation and activation of JAK2 in VSMCs.

Original languageEnglish (US)
Pages (from-to)2381-2388
Number of pages8
JournalDiabetes
Volume52
Issue number9
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

Janus Kinase 2
Vascular Smooth Muscle
Angiotensin II
Smooth Muscle Myocytes
Cell Proliferation
Reactive Oxygen Species
Phosphorylation
Phospholipase D
Tyrosine
Signal Transduction
Intercellular Signaling Peptides and Proteins
Advanced Glycosylation End Product-Specific Receptor
Ligands
Glucose
Peptides

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

S100b-RAGE-mediated augmentation of angiotensin II-induced activation of JAK2 in vascular smooth muscle cells is dependent on PLD2. / Shaw, Sean S.; Schmidt, Ann Marie; Banes, Amy K.; Wang, Xiaodan; Stern, David; Marrero, Mario B.

In: Diabetes, Vol. 52, No. 9, 01.09.2003, p. 2381-2388.

Research output: Contribution to journalArticle

Shaw, Sean S. ; Schmidt, Ann Marie ; Banes, Amy K. ; Wang, Xiaodan ; Stern, David ; Marrero, Mario B. / S100b-RAGE-mediated augmentation of angiotensin II-induced activation of JAK2 in vascular smooth muscle cells is dependent on PLD2. In: Diabetes. 2003 ; Vol. 52, No. 9. pp. 2381-2388.
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AU - Stern, David

AU - Marrero, Mario B.

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