Satellite cell activation in the stretch-enlarged anterior latissimus dorsi muscle of the adult quail

P. K. Winchester, M. E. Davis, Stephen Alway, W. J. Gonyea

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Abstract

Satellite cell activity was examined in the stretch-enlarged anterior latissimus dorsi muscle (ALD) of the adult quail. Thirty-seven birds had a weight equal to 10% of their body mass attached to one wing while the contralateral wing served as an intra-animal control. At various time intervals after application of the wing weight (from 1 to 30 days), the birds were injected with tritiated thymidine and killed 1 h later. Stretched muscle length was greater by day 1 and mass by day 3 when compared with the contralateral muscle. Satellite cells actively synthesizing DNA were quantitated in fiber segments of the control and stretched ALD. A minimum of 1,500 muscle nuclei (satellite cell nuclei and myonuclei) were counted in each muscle. Labeling in stretched muscle was expressed by the percent labeled nuclei per total nuclei counted. Satellite cell labeling was initiated by day 1, peaked between days 3 and 7, and was not statistically different from control values at day 30. These results demonstrate that satellite cells are induced to enter the cell cycle in the stretch-enlarged ALD muscle from the adult quail, and the peak of proliferative activity is within the first week of stretch.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume260
Issue number2 29-2
StatePublished - Mar 13 1991

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Quail
Superficial Back Muscles
Muscles
Birds
Weights and Measures
Cell Nucleus
Thymidine
Cell Cycle
DNA

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Satellite cell activation in the stretch-enlarged anterior latissimus dorsi muscle of the adult quail. / Winchester, P. K.; Davis, M. E.; Alway, Stephen; Gonyea, W. J.

In: American Journal of Physiology - Cell Physiology, Vol. 260, No. 2 29-2, 13.03.1991.

Research output: Contribution to journalArticle

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