Second attempts at mobilization of peripheral blood stem cells in patients with initial low CD34+ cell yields

Charles H. Weaver, Kurt Tauer, Bo Zhen, Lee Schwartzberg, Bonni Hazelton, Zebulon Weaver, Lawrence Lewkow, Cathy Allen, Kevin Longin, C. Dean Buckner

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to determine the effectiveness of second mobilization strategies in patients who yielded <2.5 × 10 6 CD34+ PBSC/kg after initial mobilization. Repeat mobilization attempts were made with chemotherapy and G-CSF (n = 61) or G-CSF alone (n = 58) in patients who failed initial mobilization with chemotherapy and G-CSF (n = 92) or G-CSF alone (n = 27). A median of 0.27 × 10 6 CD34+ cells/kg per apheresis was collected after the second mobilization, compared with 0.16 with initial harvests (p = 0.0001). Forty-eight percent achieved a target CD34+ cell dose ≥2.5 × 10 6 /kg when harvests from the first and second mobilizations were combined. Fifteen of 17 patients (88%) with ≥1.5 × 10 6 CD34+ cells/kg harvested after first mobilization had ≥2.5 × 10 6 CD34+ cells/kg collected when first and second harvests were combined, as compared with 42 of 102 (41%) achieving <1.5 × 10 6 CD34+ cells/kg with first PBSC harvests (p = 0.0001). Second mobilizations with chemotherapy and G-CSF or G-CSF alone resulted in similar CD34+ cell yields. Toxicities of second mobilizations were comparable with those of first mobilizations. Seventy-nine patients (66%) received high-dose chemotherapy with PBSC support, with recovery of neutrophils and platelets in a median of 11 and 15 days, respectively. Transplant-related mortality was 4%, and event-free survival at 2 years was 0.34. It was concluded that second mobilization attempts in patients who fail to achieve ≥2.5 × 10 6 CD34+ cells/kg on initial mobilization were successful in 48% of patients. G-CSF alone was as effective as chemotherapy plus G-CSF in mobilizing CD34+ cells and was associated with less morbidity.

Original languageEnglish (US)
Pages (from-to)241-249
Number of pages9
JournalJournal of Hematotherapy and Stem Cell Research
Volume7
Issue number3
StatePublished - Jun 1 1998

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Granulocyte Colony-Stimulating Factor
Drug Therapy
Blood Component Removal
Peripheral Blood Stem Cells
Disease-Free Survival
Neutrophils
Blood Platelets
Morbidity
Transplants
Mortality

All Science Journal Classification (ASJC) codes

  • Immunology
  • Hematology

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Second attempts at mobilization of peripheral blood stem cells in patients with initial low CD34+ cell yields. / Weaver, Charles H.; Tauer, Kurt; Zhen, Bo; Schwartzberg, Lee; Hazelton, Bonni; Weaver, Zebulon; Lewkow, Lawrence; Allen, Cathy; Longin, Kevin; Buckner, C. Dean.

In: Journal of Hematotherapy and Stem Cell Research, Vol. 7, No. 3, 01.06.1998, p. 241-249.

Research output: Contribution to journalArticle

Weaver, CH, Tauer, K, Zhen, B, Schwartzberg, L, Hazelton, B, Weaver, Z, Lewkow, L, Allen, C, Longin, K & Buckner, CD 1998, 'Second attempts at mobilization of peripheral blood stem cells in patients with initial low CD34+ cell yields', Journal of Hematotherapy and Stem Cell Research, vol. 7, no. 3, pp. 241-249.
Weaver, Charles H. ; Tauer, Kurt ; Zhen, Bo ; Schwartzberg, Lee ; Hazelton, Bonni ; Weaver, Zebulon ; Lewkow, Lawrence ; Allen, Cathy ; Longin, Kevin ; Buckner, C. Dean. / Second attempts at mobilization of peripheral blood stem cells in patients with initial low CD34+ cell yields. In: Journal of Hematotherapy and Stem Cell Research. 1998 ; Vol. 7, No. 3. pp. 241-249.
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AU - Allen, Cathy

AU - Longin, Kevin

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