Secretory activity of bovine ovarian granulosa cells transfected with sense and antisense insulin-like growth factor (IGF) binding protein-3 and the response to IGF-I, GH, LH, oxytocin and oestradiol

A. V. Sirotkin, A. V. Makarevich, Mark Corkins, J. Kotwica, H. B. Kwon, J. Bulla, L. Hetényi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aim of our in vitro experiments was to examine if IGF binding protein (IGFBP)-3 is involved in control of bovine ovarian secretory activity. For this purpose we performed the transfection of bovine granulosa cells with cDNA sense and antisense constructs increasing or inhibiting IGFBP-3 synthesis. The release of IGFBP-3, progesterone, oxytocin, IGF-I and prostaglandins F (PGF) and E (PGE) by control and transfected cells was compared. The transfected ovarian cells were cultured with and without bLH (100 ng/ml), bGH (100 ng/ml), IGF-I (10 ng/ml), oxytocin (10 ng/ml) and oestradiol-17β (100 ng/ml). The concentration of IGFBP-3 produced was assessed using ligand and western blotting and secretion of progesterone, oxytocin, IGF-I, PGF and PGE was evaluated using RIA/IRMA techniques. Transfection of cells with the sense IGFBP-3 cDNA construct resulted in the expected increase in IGFBP-3 release, whereas the antisense IGFBP-3 construct induced the expected reduction in IGFBP-3 output. The granulosa cells transfected to overexpress IGFBP-3 had an increase in IGF-I, PGF and PGE release, and a decrease in basal and hormone- or growth factor-induced accumulation of progesterone and oxytocin. The granulosa cells transfected to have reduced IGFBP-3 expression gave primarily significant opposite findings. The present results suggest the involvement of IGFBP-3 in control of bovine ovarian steroid, peptide hormone, growth factor and prostaglandin release. IGFBP-3 is a physiological stimulator of IGF-I and prostaglandin release and an inhibitor of steroid and peptide hormone output.

Original languageEnglish (US)
Pages (from-to)329-338
Number of pages10
JournalJournal of Molecular Endocrinology
Volume27
Issue number3
DOIs
StatePublished - Dec 22 2001
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor Binding Protein 3
Granulosa Cells
Oxytocin
Insulin-Like Growth Factor I
Estradiol
Prostaglandins F
Prostaglandins E
Progesterone
Peptide Hormones
Transfection
Intercellular Signaling Peptides and Proteins
Complementary DNA
Steroids
Prostaglandin Antagonists
Prostaglandins
Cultured Cells
Western Blotting

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Molecular Biology
  • Endocrinology

Cite this

Secretory activity of bovine ovarian granulosa cells transfected with sense and antisense insulin-like growth factor (IGF) binding protein-3 and the response to IGF-I, GH, LH, oxytocin and oestradiol. / Sirotkin, A. V.; Makarevich, A. V.; Corkins, Mark; Kotwica, J.; Kwon, H. B.; Bulla, J.; Hetényi, L.

In: Journal of Molecular Endocrinology, Vol. 27, No. 3, 22.12.2001, p. 329-338.

Research output: Contribution to journalArticle

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