Secretory phospholipase A2 group IIA enhances the metabolic rate and increases glucose utilization in response to thyroid hormone

Michael S. Kuefner, Xiong Deng, Erin J. Stephenson, Kevin Pham, Edwards Park

Research output: Contribution to journalArticle

Abstract

Secretory phospholipase A2 group IIA (PLA2G2A) is a phospholipase which has a role in inflammation, atherogenesis, and host defense. Previously, we found that PLA2G2A protects mice on high-fat diets from weight gain and insulin resistance. Here, we examined the regulation of PLA2G2A and the metabolic changes that occur in response to variations in thyroid status. In particular, the impact of PLA2G2A on the brown adipose tissue (BAT) thermogenic gene expression was explored.We induced hypothyroidismin C57BL/6 and PLA2G2A-overexpressing (IIA+) mice over a 10 wk period or treated them with thyroid hormone (T3) for 5 wk. There were no significant changes in PLA2G2A abundance in response to thyroid status. The energy expenditure of hypothyroid IIA+ mice did not increase; however, the energy expenditure, substrate utilization, insulin sensitivity, and glucose tolerance were all elevated in the IIA+ mice given T3. Moreover, white adipocytes from IIA+ mice were much more prone to "beiging," including increased expression of brown adipose thermogenic markers such as uncoupling protein 1 (UCP1), PR domain containing 16, and early B cell factor 2. Finally, the BAT of IIA+ mice had increased UCP1 and other proteins indicative of mitochondrial uncoupling and nonshivering adaptive thermogenesis. These data reveal a novel role for PLA2G2A on adipose tissue thermogenesis depending on thyroid status.

Original languageEnglish (US)
Pages (from-to)738-749
Number of pages12
JournalFASEB Journal
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2019

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Group II Phospholipases A2
Thyroid Hormones
Tissue
Glucose
Thermogenesis
Insulin
Thyroid Gland
Brown Adipose Tissue
Phospholipases
Mitochondrial Proteins
Triiodothyronine
Nutrition
Gene expression
Energy Metabolism
Insulin Resistance
Proteins
White Adipocytes
Fats
Cells
High Fat Diet

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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Secretory phospholipase A2 group IIA enhances the metabolic rate and increases glucose utilization in response to thyroid hormone. / Kuefner, Michael S.; Deng, Xiong; Stephenson, Erin J.; Pham, Kevin; Park, Edwards.

In: FASEB Journal, Vol. 33, No. 1, 01.01.2019, p. 738-749.

Research output: Contribution to journalArticle

Kuefner, Michael S. ; Deng, Xiong ; Stephenson, Erin J. ; Pham, Kevin ; Park, Edwards. / Secretory phospholipase A2 group IIA enhances the metabolic rate and increases glucose utilization in response to thyroid hormone. In: FASEB Journal. 2019 ; Vol. 33, No. 1. pp. 738-749.
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