Secretory phospholipase A2 group IIA modulates insulin sensitivity and metabolism

Michael S. Kuefner, Kevin Pham, Jeanna R. Redd, Erin Stephenson, Innocence Harvey, Xiong Deng, Dave Bridges, Eric Boilard, Marshall Elam, Edwards Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Secretory phospholipase A2 group IIA (PLA2G2A) is a member of a family of secretory phospholipases that have been implicated in inflammation, atherogenesis, and antibacterial actions. Here, we evaluated the role of PLA2G2A in the metabolic response to a high fat diet. C57BL/6 (BL/6) mice do not express PLA2g2a due to a frameshift mutation. We fed BL/6 mice expressing the human PLA2G2A gene (IIA+ mice) a fat diet and assessed the physiologic response. After 10 weeks on the high fat diet, the BL/6 mice were obese, but the IIA+ mice did not gain weight or accumulate lipid. The lean mass in chow-and high fat-fed IIA+ mice was constant and similar to the BL/6 mice on a chow diet. Surprisingly, the IIA+ mice had an elevated metabolic rate, which was not due to differences in physical activity. The IIA+ mice were more insulin sensitive and glucose tolerant than the BL/6 mice, even when the IIA+ mice were provided the high fat diet. The IIA+ mice had increased expression of uncoupling protein 1 (UCP1), sirtuin 1 (SIRT1), and PPAR coactivator 1 (PGC-1) in brown adipose tissue (BAT), suggesting that PLA2G2A activates mitochondrial uncoupling in BAT. Our data indicate that PLA2G2A has a previously undiscovered impact on insulin sensitivity and metabolism.

Original languageEnglish (US)
Pages (from-to)1822-1833
Number of pages12
JournalJournal of lipid research
Volume58
Issue number9
DOIs
StatePublished - Jan 1 2017

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Group II Phospholipases A2
Nutrition
Metabolism
Insulin Resistance
Fats
Insulin
Sirtuin 1
High Fat Diet
Tissue
Peroxisome Proliferator-Activated Receptors
Phospholipases
Brown Adipose Tissue
Genes
Lipids
Glucose
Diet
Obese Mice
Frameshift Mutation
Weight Gain
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Secretory phospholipase A2 group IIA modulates insulin sensitivity and metabolism. / Kuefner, Michael S.; Pham, Kevin; Redd, Jeanna R.; Stephenson, Erin; Harvey, Innocence; Deng, Xiong; Bridges, Dave; Boilard, Eric; Elam, Marshall; Park, Edwards.

In: Journal of lipid research, Vol. 58, No. 9, 01.01.2017, p. 1822-1833.

Research output: Contribution to journalArticle

Kuefner, MS, Pham, K, Redd, JR, Stephenson, E, Harvey, I, Deng, X, Bridges, D, Boilard, E, Elam, M & Park, E 2017, 'Secretory phospholipase A2 group IIA modulates insulin sensitivity and metabolism', Journal of lipid research, vol. 58, no. 9, pp. 1822-1833. https://doi.org/10.1194/jlr.M076141
Kuefner, Michael S. ; Pham, Kevin ; Redd, Jeanna R. ; Stephenson, Erin ; Harvey, Innocence ; Deng, Xiong ; Bridges, Dave ; Boilard, Eric ; Elam, Marshall ; Park, Edwards. / Secretory phospholipase A2 group IIA modulates insulin sensitivity and metabolism. In: Journal of lipid research. 2017 ; Vol. 58, No. 9. pp. 1822-1833.
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