Segregation of Histone Fractions from Purified Rat Pancreas Nuclei by Isoelectric Focusing

Levy Kopelovich, Gloria Wolfe, Lawrence Pfeffer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The present report describes the purification of nuclei from rat pancreas and the use of isoelectric focusing for the segregation of histone fractions, a heterogeneous mixture with respect to charge. This procedure employs gels of 5% polyacrylamide as support matrices, in 6.25 M urea, and spans a pH range of 7.5-10.8. It was, therefore, found adequate for study of the microheterogeneity of histones. The banding pattern of histone fractions in pancreas development using electrofocusing is presented.

Original languageEnglish (US)
Pages (from-to)400-406
Number of pages7
JournalProceedings of the Society for Experimental Biology and Medicine
Volume151
Issue number2
DOIs
StatePublished - Jan 1 1976

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Isoelectric Focusing
Histones
Rats
Pancreas
Purification
Urea
Gels

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Segregation of Histone Fractions from Purified Rat Pancreas Nuclei by Isoelectric Focusing. / Kopelovich, Levy; Wolfe, Gloria; Pfeffer, Lawrence.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 151, No. 2, 01.01.1976, p. 400-406.

Research output: Contribution to journalArticle

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