Selective Interferon-α/β Effects on Platelet-Derived Growth Factor-Stimulated Processes in Quiescent BALB/c-3T3 Fibroblasts

Igor Tamm, Toyoko Kikuchi, David Kreutter, W. Jackson Pledger, Lawrence Pfeffer

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Interferon-α/β (IFN-α/β) suppresses cell cycle activation by platelet-derived growth factor (PDGF) as well as the induction of the 31-kD (pI) and the 35-kD (pH) proteins in density-arrested BALB/c-3T3 cells.(1,2) We report that elevation of [Ca2+]i by ionomycin induces the synthesis of the 31-kD protein, but not that of the 35-kD protein. Since IFN blocks the PDGF-induced elevation of [Ca2+]i,(3) these results suggest that IFN treatment may suppress pI induction by impairing this PDGF-activated signal transduction pathway. In contrast, because ionomycin did not induce the 35-kD protein, the suppression by IFN of PDGF-induced pII appears to be mediated via a pathway distinct from that operating in the suppression of pI. In BALB/c-3T3 cells, IFN-α/β did not itself affect the turnover or de novo synthesis of inositol phospholipids and the cellular content of diacylglycerol, nor did IFN block the enhancement of these parameters by PDGF.

Original languageEnglish (US)
Pages (from-to)265-273
Number of pages9
JournalJournal of Interferon Research
Volume14
Issue number5
DOIs
StatePublished - Jan 1 1994

Fingerprint

Platelet-Derived Growth Factor
Interferons
Fibroblasts
BALB 3T3 Cells
Ionomycin
Proteins
Diglycerides
Phosphatidylinositols
Signal Transduction
Cell Cycle

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Selective Interferon-α/β Effects on Platelet-Derived Growth Factor-Stimulated Processes in Quiescent BALB/c-3T3 Fibroblasts. / Tamm, Igor; Kikuchi, Toyoko; Kreutter, David; Pledger, W. Jackson; Pfeffer, Lawrence.

In: Journal of Interferon Research, Vol. 14, No. 5, 01.01.1994, p. 265-273.

Research output: Contribution to journalArticle

@article{b03f4f836b2f4730ae9a24a52ebee0fe,
title = "Selective Interferon-α/β Effects on Platelet-Derived Growth Factor-Stimulated Processes in Quiescent BALB/c-3T3 Fibroblasts",
abstract = "Interferon-α/β (IFN-α/β) suppresses cell cycle activation by platelet-derived growth factor (PDGF) as well as the induction of the 31-kD (pI) and the 35-kD (pH) proteins in density-arrested BALB/c-3T3 cells.(1,2) We report that elevation of [Ca2+]i by ionomycin induces the synthesis of the 31-kD protein, but not that of the 35-kD protein. Since IFN blocks the PDGF-induced elevation of [Ca2+]i,(3) these results suggest that IFN treatment may suppress pI induction by impairing this PDGF-activated signal transduction pathway. In contrast, because ionomycin did not induce the 35-kD protein, the suppression by IFN of PDGF-induced pII appears to be mediated via a pathway distinct from that operating in the suppression of pI. In BALB/c-3T3 cells, IFN-α/β did not itself affect the turnover or de novo synthesis of inositol phospholipids and the cellular content of diacylglycerol, nor did IFN block the enhancement of these parameters by PDGF.",
author = "Igor Tamm and Toyoko Kikuchi and David Kreutter and Pledger, {W. Jackson} and Lawrence Pfeffer",
year = "1994",
month = "1",
day = "1",
doi = "10.1089/jir.1994.14.265",
language = "English (US)",
volume = "14",
pages = "265--273",
journal = "Journal of Interferon and Cytokine Research",
issn = "1079-9907",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

TY - JOUR

T1 - Selective Interferon-α/β Effects on Platelet-Derived Growth Factor-Stimulated Processes in Quiescent BALB/c-3T3 Fibroblasts

AU - Tamm, Igor

AU - Kikuchi, Toyoko

AU - Kreutter, David

AU - Pledger, W. Jackson

AU - Pfeffer, Lawrence

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Interferon-α/β (IFN-α/β) suppresses cell cycle activation by platelet-derived growth factor (PDGF) as well as the induction of the 31-kD (pI) and the 35-kD (pH) proteins in density-arrested BALB/c-3T3 cells.(1,2) We report that elevation of [Ca2+]i by ionomycin induces the synthesis of the 31-kD protein, but not that of the 35-kD protein. Since IFN blocks the PDGF-induced elevation of [Ca2+]i,(3) these results suggest that IFN treatment may suppress pI induction by impairing this PDGF-activated signal transduction pathway. In contrast, because ionomycin did not induce the 35-kD protein, the suppression by IFN of PDGF-induced pII appears to be mediated via a pathway distinct from that operating in the suppression of pI. In BALB/c-3T3 cells, IFN-α/β did not itself affect the turnover or de novo synthesis of inositol phospholipids and the cellular content of diacylglycerol, nor did IFN block the enhancement of these parameters by PDGF.

AB - Interferon-α/β (IFN-α/β) suppresses cell cycle activation by platelet-derived growth factor (PDGF) as well as the induction of the 31-kD (pI) and the 35-kD (pH) proteins in density-arrested BALB/c-3T3 cells.(1,2) We report that elevation of [Ca2+]i by ionomycin induces the synthesis of the 31-kD protein, but not that of the 35-kD protein. Since IFN blocks the PDGF-induced elevation of [Ca2+]i,(3) these results suggest that IFN treatment may suppress pI induction by impairing this PDGF-activated signal transduction pathway. In contrast, because ionomycin did not induce the 35-kD protein, the suppression by IFN of PDGF-induced pII appears to be mediated via a pathway distinct from that operating in the suppression of pI. In BALB/c-3T3 cells, IFN-α/β did not itself affect the turnover or de novo synthesis of inositol phospholipids and the cellular content of diacylglycerol, nor did IFN block the enhancement of these parameters by PDGF.

UR - http://www.scopus.com/inward/record.url?scp=0028556484&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028556484&partnerID=8YFLogxK

U2 - 10.1089/jir.1994.14.265

DO - 10.1089/jir.1994.14.265

M3 - Article

VL - 14

SP - 265

EP - 273

JO - Journal of Interferon and Cytokine Research

JF - Journal of Interferon and Cytokine Research

SN - 1079-9907

IS - 5

ER -