Selenium protects cerebral ischemia in rat brain mitochondria

Mubeen Ahmad Ansari, Abdullah Shafique Ahmad, Muzamil Ahmad, Sofiyan Salim, Seema Yousuf, Tauheed Ishrat, Fakhrul Islam

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Normal cellular metabolism produces oxidants that are neutralized by the cells' antioxidant enzymes and antioxidants taken from outside. An imbalance between oxidant and antioxidant has been postulated to lead to the neurodegeneration in the ischemic condition. In this study, we have demonstrated the prevention or slowdown of neuronal injury in middle cerebral artery occlusion (MCAO) by sodium selenite. Rats were pretreated with 0.05, 0.1, and 0.2 mg/kg body wt of sodium selenite for 7 d. The rats of group I (sham) and group II (ischemia) were pretreated with physiological saline for 7 d. On d 8, MCAO was induced for 2 h in the right side of brain of group II, III, IV, and V rats. Brains were dissect out after 22 h of reperfusion and washed with chilled physiological saline. The right cerebral hemisphere was used for the preparation of mitochondria. The activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, and monoamine oxidase (MAO-A and MAO-B) was depleted significantly; conversely, the activity of poly(ADP-ribosyl) polymerase was elevated significantly as compared to the sham, and the pretreatment of the animals with different doses of sodium selenite has protected the activity of these enzymes significantly. The content of glutathione was decreased significantly, whereas the level of lipid peroxidation was increased significantly in the mitochondria of MCAO as compared to the sham group, and pretreatment with different doses of sodium selenite has protected their levels significantly as compared to the MCAO group. It is concluded that selenium, which is an essential part of our diet, might be helpful in protection against neurodegeneration in cerebral ischemia.

Original languageEnglish (US)
Pages (from-to)73-86
Number of pages14
JournalBiological Trace Element Research
Volume101
Issue number1
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

Fingerprint

Sodium Selenite
Mitochondria
Middle Cerebral Artery Infarction
Selenium
Brain Ischemia
Rats
Brain
Monoamine Oxidase
Antioxidants
Oxidants
Cerebrum
Enzymes
Nutrition
Glutathione Peroxidase
Glutathione Transferase
Metabolism
Catalase
Adenosine Diphosphate
Lipid Peroxidation
Superoxide Dismutase

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry
  • Inorganic Chemistry
  • Biochemistry, medical

Cite this

Ansari, M. A., Ahmad, A. S., Ahmad, M., Salim, S., Yousuf, S., Ishrat, T., & Islam, F. (2004). Selenium protects cerebral ischemia in rat brain mitochondria. Biological Trace Element Research, 101(1), 73-86. https://doi.org/10.1385/BTER:101:1:73

Selenium protects cerebral ischemia in rat brain mitochondria. / Ansari, Mubeen Ahmad; Ahmad, Abdullah Shafique; Ahmad, Muzamil; Salim, Sofiyan; Yousuf, Seema; Ishrat, Tauheed; Islam, Fakhrul.

In: Biological Trace Element Research, Vol. 101, No. 1, 01.10.2004, p. 73-86.

Research output: Contribution to journalArticle

Ansari, MA, Ahmad, AS, Ahmad, M, Salim, S, Yousuf, S, Ishrat, T & Islam, F 2004, 'Selenium protects cerebral ischemia in rat brain mitochondria', Biological Trace Element Research, vol. 101, no. 1, pp. 73-86. https://doi.org/10.1385/BTER:101:1:73
Ansari, Mubeen Ahmad ; Ahmad, Abdullah Shafique ; Ahmad, Muzamil ; Salim, Sofiyan ; Yousuf, Seema ; Ishrat, Tauheed ; Islam, Fakhrul. / Selenium protects cerebral ischemia in rat brain mitochondria. In: Biological Trace Element Research. 2004 ; Vol. 101, No. 1. pp. 73-86.
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