Senile seminal vesicle amyloid is derived from semenogelin I

Reinhold P. Linke, Reinhild Joswig, Charles L. Murphy, Shuching Wang, Hui Zhou, Ulrich Gross, Christoph Rocken, Per Westermark, Deborah T. Weiss, Alan Solomon

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Senile seminal vesicle amyloid (SSVA), one of the most common forms of localized amyloidosis, is associated with the male aging process. Although it had been posited that the amyloidogenic component originated from exocrine cells and that, on the basis of immunohistochemistry, that the amyloid was composed of lactoferrin, the nature of SSVA was never established definitively. To address this issue, we have used our microanalytic techniques to characterize the structure of the congophilic green birefringent protein extracted from 5 such amyloid-containing specimens. Mass spectrometric analysis revealed that in all cases, the fibrils were composed mainly of polypeptide fragments identical in sequence to the N-terminal portion of the major secretory product of seminal vesicles, namely semenogelin I (SgI). Although lactoferrin was detected in 3 instances, the trace amount and seemingly intact form of this molecule indicated that it was not the amyloidogenic molecule. The SgI nature of the amyloid was confirmed through demonstration that the deposits were immunostained specifically with SgI-reactive antibodies. The results of our research provide unequivocal evidence that SSVA is derived from SgI, and we provisionally designate this form of amyloidosis as ASgI.

Original languageEnglish (US)
Pages (from-to)187-193
Number of pages7
JournalJournal of Laboratory and Clinical Medicine
Volume145
Issue number4
DOIs
StatePublished - Apr 1 2005

Fingerprint

Seminal Vesicles
Amyloid
Lactoferrin
Amyloidosis
Molecules
Demonstrations
Deposits
Aging of materials
Immunohistochemistry
Peptides
Antibodies
Research
Proteins

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Senile seminal vesicle amyloid is derived from semenogelin I. / Linke, Reinhold P.; Joswig, Reinhild; Murphy, Charles L.; Wang, Shuching; Zhou, Hui; Gross, Ulrich; Rocken, Christoph; Westermark, Per; Weiss, Deborah T.; Solomon, Alan.

In: Journal of Laboratory and Clinical Medicine, Vol. 145, No. 4, 01.04.2005, p. 187-193.

Research output: Contribution to journalArticle

Linke, RP, Joswig, R, Murphy, CL, Wang, S, Zhou, H, Gross, U, Rocken, C, Westermark, P, Weiss, DT & Solomon, A 2005, 'Senile seminal vesicle amyloid is derived from semenogelin I', Journal of Laboratory and Clinical Medicine, vol. 145, no. 4, pp. 187-193. https://doi.org/10.1016/j.lab.2005.02.002
Linke, Reinhold P. ; Joswig, Reinhild ; Murphy, Charles L. ; Wang, Shuching ; Zhou, Hui ; Gross, Ulrich ; Rocken, Christoph ; Westermark, Per ; Weiss, Deborah T. ; Solomon, Alan. / Senile seminal vesicle amyloid is derived from semenogelin I. In: Journal of Laboratory and Clinical Medicine. 2005 ; Vol. 145, No. 4. pp. 187-193.
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