Sensitive method of caspase-3 detection in single stem cell

Eva Adamová, Karel Klepárník, Eva Matalová

Research output: Contribution to journalArticle

Abstract

Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemi-luminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10-15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.

Original languageEnglish (US)
JournalChemicke Listy
Volume107
Issue number3
StatePublished - 2013
Externally publishedYes

Fingerprint

Stem cells
Caspase 3
Photons
Photomultipliers
Caspases
Luciferases
Luminescence

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Sensitive method of caspase-3 detection in single stem cell. / Adamová, Eva; Klepárník, Karel; Matalová, Eva.

In: Chemicke Listy, Vol. 107, No. 3, 2013.

Research output: Contribution to journalArticle

Adamová, E, Klepárník, K & Matalová, E 2013, 'Sensitive method of caspase-3 detection in single stem cell', Chemicke Listy, vol. 107, no. 3.
Adamová, Eva ; Klepárník, Karel ; Matalová, Eva. / Sensitive method of caspase-3 detection in single stem cell. In: Chemicke Listy. 2013 ; Vol. 107, No. 3.
@article{a3a751ddfa844c9099fb5dd3438dd8f8,
title = "Sensitive method of caspase-3 detection in single stem cell",
abstract = "Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemi-luminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10-15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.",
author = "Eva Adamov{\'a} and Karel Klep{\'a}rn{\'i}k and Eva Matalov{\'a}",
year = "2013",
language = "English (US)",
volume = "107",
journal = "Chemicke Listy",
issn = "0009-2770",
publisher = "Czech Society of Chemical Engineering",
number = "3",

}

TY - JOUR

T1 - Sensitive method of caspase-3 detection in single stem cell

AU - Adamová, Eva

AU - Klepárník, Karel

AU - Matalová, Eva

PY - 2013

Y1 - 2013

N2 - Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemi-luminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10-15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.

AB - Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemi-luminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10-15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.

UR - http://www.scopus.com/inward/record.url?scp=84891773228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891773228&partnerID=8YFLogxK

M3 - Article

VL - 107

JO - Chemicke Listy

JF - Chemicke Listy

SN - 0009-2770

IS - 3

ER -