Separate ultrastructural distributions of neurophysin and C-terminal glycopeptide within dense core vesicles in rat neural lobe

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Abstract

The distributions of the neurohypophysial prohormone products neurophysin and C-terminal glycopeptide within dense core vesicles were investigated at the ultrastructural level using quantitative immunogold labeling of rapidly frozen rat neural lobes. In vasopressin vesicles, gold particles after labeling for the glycopeptide wer found significantly closer to membrane (x = 16 nm) than were particles after labeling for neurophysin (x = 32 nm). This distribution supports a previous histochemical localization of glycopeptide to the rim of vasopressin vesicle and suggests there is morphological segregation of prohormone products within the vesicle.

Original languageEnglish (US)
Pages (from-to)144-148
Number of pages5
JournalBrain Research
Volume562
Issue number1
DOIs
StatePublished - Oct 18 1991

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Neurophysins
Posterior Pituitary Gland
Glycopeptides
Secretory Vesicles
Vasopressins
Gold
Membranes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Separate ultrastructural distributions of neurophysin and C-terminal glycopeptide within dense core vesicles in rat neural lobe. / Armstrong, William; Tian, Min.

In: Brain Research, Vol. 562, No. 1, 18.10.1991, p. 144-148.

Research output: Contribution to journalArticle

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