Sequence analysis of mouse tyrosinase cDNA and the effect of melanotropin on its gene expression

Byoung S. Kwon, Mark Wakulchik, Asifa K. Haq, Ruth Halaban, Daniel Kestler

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Using human tyrosinase cDNA as a probe, a mouse tyrosinase cDNA clone representing approximately 75% of the tyrosinase coding region and a mouse genomic clone which includes the tyrosinase 5′ coding sequences were isolated: nucleotide and deduced amino acid sequence of the mouse tyrosinase gene were determined from these clones. The predicted amino acid sequence revealed that the mouse tyrosinase is composed of 533 amino acids with a molecular weight of 60,536. The deduced protein contains 6 potential N-glycosylation sites, two cysteine- and two histidine-rich regions which may serve as copper-binding sites, a potential signal and transmembrane sequences. The mouse and human tyrosinase nucleotide and deduced amino acid sequences are ∼81% homologous. The level of mouse tyrosinase mRNA was elevated after stimulation of Cloudman S-91 melanoma cells with melanotropin and isobutylmethylxanthine and the level of transcript reflected that of tyrosinase activity and melanin content in the cells.

Original languageEnglish (US)
Pages (from-to)1301-1309
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume153
Issue number3
DOIs
StatePublished - Jun 30 1988
Externally publishedYes

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Melanocyte-Stimulating Hormones
Monophenol Monooxygenase
Gene expression
Sequence Analysis
Complementary DNA
Gene Expression
Amino Acids
Clone Cells
Amino Acid Sequence
Nucleotides
Glycosylation
Amino Acid Sequence Homology
Melanins
Protein Sorting Signals
Histidine
Membrane Potentials
Cysteine
Copper
Melanoma
Genes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sequence analysis of mouse tyrosinase cDNA and the effect of melanotropin on its gene expression. / Kwon, Byoung S.; Wakulchik, Mark; Haq, Asifa K.; Halaban, Ruth; Kestler, Daniel.

In: Biochemical and Biophysical Research Communications, Vol. 153, No. 3, 30.06.1988, p. 1301-1309.

Research output: Contribution to journalArticle

Kwon, Byoung S. ; Wakulchik, Mark ; Haq, Asifa K. ; Halaban, Ruth ; Kestler, Daniel. / Sequence analysis of mouse tyrosinase cDNA and the effect of melanotropin on its gene expression. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 153, No. 3. pp. 1301-1309.
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