Sequence of endothelial signaling during lung expansion

Maimaiti T. Yiming, Kaushik Parthasarathi, Andrew C. Issekutz, Sunita Bhattacharya

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although high tidal volume ventilation exacerbates lung injury, the mechanisms underlying the inflammatory response are not clear. Here, we exposed isolated lungs to high or low tidal volume ventilation, while perfusing lungs with whole blood, or blood depleted of leukocytes and platelets. Then, we determined signaling responses in freshly isolated lung endothelial cells by means of immunoblotting and immunofluorescence approaches. In depleted blood perfusion, high tidal volume induced modest increases in both P-selectin expression on the endothelial surface, and in endothelial protein tyrosine phosphorylation. Both high tidal volume-induced responses were markedly enhanced in the presence of whole blood perfusion. However, a P-selectin-blocking antibody given together with whole blood perfusion inhibited the responses down to levels corresponding to those for depleted blood perfusion. These findings indicate that the full proinflammatory response occurs in two stages. First, lung distension causes modest endothelial activation. Second, subsequent endothelial-inflammatory cell interactions augment P-selectin expression and tyrosine phosphorylation. We conclude that interactions of circulating inflammatory cells with P-selectin critically determine proinflammatory endothelial activation during high tidal volume ventilation.

Original languageEnglish (US)
Pages (from-to)549-554
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Volume33
Issue number6
DOIs
StatePublished - Dec 1 2005

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Tidal Volume
P-Selectin
Blood
Lung
Perfusion
Ventilation
Phosphorylation
Endothelial cells
Tyrosine
Endothelial Cells
Chemical activation
Blocking Antibodies
Lung Injury
Platelets
Immunoblotting
Cell Communication
Fluorescent Antibody Technique
Leukocytes
Blood Platelets
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Sequence of endothelial signaling during lung expansion. / Yiming, Maimaiti T.; Parthasarathi, Kaushik; Issekutz, Andrew C.; Bhattacharya, Sunita.

In: American journal of respiratory cell and molecular biology, Vol. 33, No. 6, 01.12.2005, p. 549-554.

Research output: Contribution to journalArticle

Yiming, Maimaiti T. ; Parthasarathi, Kaushik ; Issekutz, Andrew C. ; Bhattacharya, Sunita. / Sequence of endothelial signaling during lung expansion. In: American journal of respiratory cell and molecular biology. 2005 ; Vol. 33, No. 6. pp. 549-554.
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