Sequence of protective epitopes of streptococcal M proteins shared with cardiac sarcolemmal membranes

S. J. Sargent, E. H. Beachey, C. E. Corbett, James Dale

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Synthetic peptide fragments spanning the entire amino acid sequence of pep M5 were used to detect epitopes cross-reactive with heart tissue components other than myosin. Heart-cross-reactive pep M5 antibodies were affinity purified by absorption to and elution from purified sarcolemmal membranes. Only one of the synthetic peptides, SM5(164-197)C, inhibited reactivity of the affinity-purified antibodies with pep M5 by ELISA. SM5(164-197)C linked to KLH evoked both opsonic and heart-cross-reactive antibodies in rabbits. In addition to type 5, the immune sera opsonized M types 6, 18, 19, and 49 streptococci. The antisera reacted strongly with isolated cardiac sarcolemmal membranes by immunofluorescence. In Western blots of cardiac tissue, the anti-SM5(164-197)C reacted with a 40 kDa protein but not with myosin. The reaction was inhibited by pep M5 and SM5(164-197)C but not by any of the other peptides spanning pep M5. The cross-reactive anti-SM5(164-197)C affinity purified on sarcolemmal membranes opsonized types 5, 6, and 19 but not type 24 streptococci. These results indicate that SM5(164-197)C contains heart-cross-reactive, opsonic epitopes that are shared among heterologous serotypes of group A streptococci.

Original languageEnglish (US)
Pages (from-to)1285-1290
Number of pages6
JournalJournal of Immunology
Volume139
Issue number4
StatePublished - Jan 1 1987
Externally publishedYes

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Epitopes
Streptococcus
Antibody Affinity
Membranes
Myosins
Immune Sera
Peptides
Peptide Fragments
Fluorescent Antibody Technique
Amino Acid Sequence
Western Blotting
Enzyme-Linked Immunosorbent Assay
Rabbits
streptococcal M protein
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Sequence of protective epitopes of streptococcal M proteins shared with cardiac sarcolemmal membranes. / Sargent, S. J.; Beachey, E. H.; Corbett, C. E.; Dale, James.

In: Journal of Immunology, Vol. 139, No. 4, 01.01.1987, p. 1285-1290.

Research output: Contribution to journalArticle

Sargent, S. J. ; Beachey, E. H. ; Corbett, C. E. ; Dale, James. / Sequence of protective epitopes of streptococcal M proteins shared with cardiac sarcolemmal membranes. In: Journal of Immunology. 1987 ; Vol. 139, No. 4. pp. 1285-1290.
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