Sequential loss of loci in thymine-starved Bacillus subtilis 168 cells. Evidence for a circular chromosome

Ramareddy Guntaka, Harvard Reiter

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Thymine starvation of Bacillus subtilis 168 thyA, thyB cells causes the sequential loss of transforming activities of loci behind the DNA replicating point. DNA synthesis in cells synchronized by germinating spores was allowed to proceed beyond the adenine U6 locus. Upon starvation these cells lost first adenine U6, then adenine U16, then methionine, and then tryptophan-2 transforming activities. Transforming activity of leucine, which is in the middle of the chromosome, was not lost unless DNA replication was allowed to proceed beyond the leucine locus. This sequential loss of loci behind the replicating point is consistent with previous experiments showing the sequential loss of DNA behind the replicating points in thymine-starved cells. The uninterrupted loss of loci on either side of the point of initiation of DNA synthesis, is taken as evidence for the continuity of the chromosome across this point, i.e. for a physical connection between the beginning and end of the chromosome.

Original languageEnglish (US)
Pages (from-to)525-532
Number of pages8
JournalJournal of Molecular Biology
Volume50
Issue number2
DOIs
StatePublished - Jun 14 1970

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Thymine
Bacillus subtilis
Adenine
Chromosomes
DNA
Starvation
Leucine
Spores
DNA Replication
Tryptophan
Methionine

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Sequential loss of loci in thymine-starved Bacillus subtilis 168 cells. Evidence for a circular chromosome. / Guntaka, Ramareddy; Reiter, Harvard.

In: Journal of Molecular Biology, Vol. 50, No. 2, 14.06.1970, p. 525-532.

Research output: Contribution to journalArticle

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