Serologic and chemical differentiation of human λIII light chain variable regions

M. Eulitz, C. Murphy, D. T. Weiss, Alan Solomon

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Human λ L chains of a major Vλ subgroup, Vλ(III), have been differentiated serologically and chemically into three Vλ(III) sub-subgroups designated Vλ(IIIa), Vλ(IIIb), and Vλ(IIIc). Antisera prepared against λIII Bence Jones proteins were obtained that recognized distinctive Vλ(III)-related epitopes expressed by monoclonal λIII L chains. After appropriate absorption, these reagents were rendered specific for three distinct populations of λIII proteins-λIIIa, λIIIb, and λIIIc. The antisera were used in comparative immunodiffusion analyses of 28 monoclonal λIII L chains, 10 of which were classified as λIIIa, 4 as λIIIb, and 14 as λIIIc. The isotypic nature of the three λIII sub-subgroups was demonstrated serologically through analyses of λ-chains derived from the serum IgG molecules of normal individuals. The amino acid sequences of five serologically classified λIII chains, which included members of the three Vλ(III) sub-subgroups, had been previously determined. This information, in addition to our establishment of the complete (or virtually complete) V region sequence of 15 and the partial sequence of eight other λIIIa, λIIIb, and λIIIc proteins, made it possible to correlate chemical data with serologic classification. Proteins within each of the three serologically-classified λIII sub-subgroups typically possessed a high degree (~83%) of intra-sub-subgroup sequence homology that included both framework and complimentarity determining region residues. Furthermore, within the framework and complimentarity determining regions, sub-subgroup-specific residues were identified. Taken together, these data reveal that the human Vλ(III) genome consists of (at least) three distinct Vλ(IIIa), Vλ(IIIb), and Vλ(IIIc) germline genes that encode for λIIIa, λIIIb, and λIIIc L chains, respectively.

Original languageEnglish (US)
Pages (from-to)3091-3096
Number of pages6
JournalJournal of Immunology
Volume146
Issue number9
StatePublished - Jan 1 1991

Fingerprint

Light
Immune Sera
Bence Jones Protein
Proteins
Immunodiffusion
Sequence Homology
Epitopes
Amino Acid Sequence
Immunoglobulin G
Genome
Serum
Population
Genes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Serologic and chemical differentiation of human λIII light chain variable regions. / Eulitz, M.; Murphy, C.; Weiss, D. T.; Solomon, Alan.

In: Journal of Immunology, Vol. 146, No. 9, 01.01.1991, p. 3091-3096.

Research output: Contribution to journalArticle

Eulitz, M. ; Murphy, C. ; Weiss, D. T. ; Solomon, Alan. / Serologic and chemical differentiation of human λIII light chain variable regions. In: Journal of Immunology. 1991 ; Vol. 146, No. 9. pp. 3091-3096.
@article{c33e35f5b4244be2a859b908217c50c3,
title = "Serologic and chemical differentiation of human λIII light chain variable regions",
abstract = "Human λ L chains of a major Vλ subgroup, Vλ(III), have been differentiated serologically and chemically into three Vλ(III) sub-subgroups designated Vλ(IIIa), Vλ(IIIb), and Vλ(IIIc). Antisera prepared against λIII Bence Jones proteins were obtained that recognized distinctive Vλ(III)-related epitopes expressed by monoclonal λIII L chains. After appropriate absorption, these reagents were rendered specific for three distinct populations of λIII proteins-λIIIa, λIIIb, and λIIIc. The antisera were used in comparative immunodiffusion analyses of 28 monoclonal λIII L chains, 10 of which were classified as λIIIa, 4 as λIIIb, and 14 as λIIIc. The isotypic nature of the three λIII sub-subgroups was demonstrated serologically through analyses of λ-chains derived from the serum IgG molecules of normal individuals. The amino acid sequences of five serologically classified λIII chains, which included members of the three Vλ(III) sub-subgroups, had been previously determined. This information, in addition to our establishment of the complete (or virtually complete) V region sequence of 15 and the partial sequence of eight other λIIIa, λIIIb, and λIIIc proteins, made it possible to correlate chemical data with serologic classification. Proteins within each of the three serologically-classified λIII sub-subgroups typically possessed a high degree (~83{\%}) of intra-sub-subgroup sequence homology that included both framework and complimentarity determining region residues. Furthermore, within the framework and complimentarity determining regions, sub-subgroup-specific residues were identified. Taken together, these data reveal that the human Vλ(III) genome consists of (at least) three distinct Vλ(IIIa), Vλ(IIIb), and Vλ(IIIc) germline genes that encode for λIIIa, λIIIb, and λIIIc L chains, respectively.",
author = "M. Eulitz and C. Murphy and Weiss, {D. T.} and Alan Solomon",
year = "1991",
month = "1",
day = "1",
language = "English (US)",
volume = "146",
pages = "3091--3096",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",

}

TY - JOUR

T1 - Serologic and chemical differentiation of human λIII light chain variable regions

AU - Eulitz, M.

AU - Murphy, C.

AU - Weiss, D. T.

AU - Solomon, Alan

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Human λ L chains of a major Vλ subgroup, Vλ(III), have been differentiated serologically and chemically into three Vλ(III) sub-subgroups designated Vλ(IIIa), Vλ(IIIb), and Vλ(IIIc). Antisera prepared against λIII Bence Jones proteins were obtained that recognized distinctive Vλ(III)-related epitopes expressed by monoclonal λIII L chains. After appropriate absorption, these reagents were rendered specific for three distinct populations of λIII proteins-λIIIa, λIIIb, and λIIIc. The antisera were used in comparative immunodiffusion analyses of 28 monoclonal λIII L chains, 10 of which were classified as λIIIa, 4 as λIIIb, and 14 as λIIIc. The isotypic nature of the three λIII sub-subgroups was demonstrated serologically through analyses of λ-chains derived from the serum IgG molecules of normal individuals. The amino acid sequences of five serologically classified λIII chains, which included members of the three Vλ(III) sub-subgroups, had been previously determined. This information, in addition to our establishment of the complete (or virtually complete) V region sequence of 15 and the partial sequence of eight other λIIIa, λIIIb, and λIIIc proteins, made it possible to correlate chemical data with serologic classification. Proteins within each of the three serologically-classified λIII sub-subgroups typically possessed a high degree (~83%) of intra-sub-subgroup sequence homology that included both framework and complimentarity determining region residues. Furthermore, within the framework and complimentarity determining regions, sub-subgroup-specific residues were identified. Taken together, these data reveal that the human Vλ(III) genome consists of (at least) three distinct Vλ(IIIa), Vλ(IIIb), and Vλ(IIIc) germline genes that encode for λIIIa, λIIIb, and λIIIc L chains, respectively.

AB - Human λ L chains of a major Vλ subgroup, Vλ(III), have been differentiated serologically and chemically into three Vλ(III) sub-subgroups designated Vλ(IIIa), Vλ(IIIb), and Vλ(IIIc). Antisera prepared against λIII Bence Jones proteins were obtained that recognized distinctive Vλ(III)-related epitopes expressed by monoclonal λIII L chains. After appropriate absorption, these reagents were rendered specific for three distinct populations of λIII proteins-λIIIa, λIIIb, and λIIIc. The antisera were used in comparative immunodiffusion analyses of 28 monoclonal λIII L chains, 10 of which were classified as λIIIa, 4 as λIIIb, and 14 as λIIIc. The isotypic nature of the three λIII sub-subgroups was demonstrated serologically through analyses of λ-chains derived from the serum IgG molecules of normal individuals. The amino acid sequences of five serologically classified λIII chains, which included members of the three Vλ(III) sub-subgroups, had been previously determined. This information, in addition to our establishment of the complete (or virtually complete) V region sequence of 15 and the partial sequence of eight other λIIIa, λIIIb, and λIIIc proteins, made it possible to correlate chemical data with serologic classification. Proteins within each of the three serologically-classified λIII sub-subgroups typically possessed a high degree (~83%) of intra-sub-subgroup sequence homology that included both framework and complimentarity determining region residues. Furthermore, within the framework and complimentarity determining regions, sub-subgroup-specific residues were identified. Taken together, these data reveal that the human Vλ(III) genome consists of (at least) three distinct Vλ(IIIa), Vλ(IIIb), and Vλ(IIIc) germline genes that encode for λIIIa, λIIIb, and λIIIc L chains, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0025856072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025856072&partnerID=8YFLogxK

M3 - Article

VL - 146

SP - 3091

EP - 3096

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 9

ER -