Serologic crossreactions among primate immunoglobulins

Michael H. Shearer, Fred J. Stevens, F. A. Westholm, Hal B. Jenson, Tran C. Chanh, Kenneth D. Carey, Gary L. White, Alan Solomon, Ronald C. Kennedy

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have generated and characterized 50 murine monoclonal antibodies (mAb) specific for baboon IgG. We examined crossreactivity of these mAb to baboon IgM and immunoglobulin (Ig) of various other primates including human, chimpanzee, rhesus monkey, cynomologous monkey, and African green monkey. Those mAB that crossreacted with human IgG were further examined using myeloma proteins for specificity to human Ig subclasses. One mAB crossreacted with all four human IgG subclasses and with human IgM. We further analyzed this reactivity utilizing Bence Jones proteins representative of various l ight (L) chain germline gene family products. This mAB reacted with Bence Jones proteins indicating the recognition of a kappa (κ) L chain specificity associated with the κi, κiii, and κIV subgroups, but not with κII. Based on the differences between κII germ line gene encoded L chains and the other κ L chain subgroups, we ascribe this reactivity to six amino acids that define a discontinuous epitope.

Original languageEnglish (US)
Pages (from-to)547-557
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume19
Issue number6
DOIs
StatePublished - Jan 1 1995

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Primates
Immunoglobulins
Bence Jones Protein
Papio
Immunoglobulin G
Immunoglobulin M
Monoclonal Antibodies
Myeloma Proteins
Cercopithecus aethiops
Pan troglodytes
Macaca mulatta
Germ Cells
Genes
Haplorhini
Epitopes
Amino Acids

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

Shearer, M. H., Stevens, F. J., Westholm, F. A., Jenson, H. B., Chanh, T. C., Carey, K. D., ... Kennedy, R. C. (1995). Serologic crossreactions among primate immunoglobulins. Developmental and Comparative Immunology, 19(6), 547-557. https://doi.org/10.1016/0145-305X(95)00026-P

Serologic crossreactions among primate immunoglobulins. / Shearer, Michael H.; Stevens, Fred J.; Westholm, F. A.; Jenson, Hal B.; Chanh, Tran C.; Carey, Kenneth D.; White, Gary L.; Solomon, Alan; Kennedy, Ronald C.

In: Developmental and Comparative Immunology, Vol. 19, No. 6, 01.01.1995, p. 547-557.

Research output: Contribution to journalArticle

Shearer, MH, Stevens, FJ, Westholm, FA, Jenson, HB, Chanh, TC, Carey, KD, White, GL, Solomon, A & Kennedy, RC 1995, 'Serologic crossreactions among primate immunoglobulins', Developmental and Comparative Immunology, vol. 19, no. 6, pp. 547-557. https://doi.org/10.1016/0145-305X(95)00026-P
Shearer MH, Stevens FJ, Westholm FA, Jenson HB, Chanh TC, Carey KD et al. Serologic crossreactions among primate immunoglobulins. Developmental and Comparative Immunology. 1995 Jan 1;19(6):547-557. https://doi.org/10.1016/0145-305X(95)00026-P
Shearer, Michael H. ; Stevens, Fred J. ; Westholm, F. A. ; Jenson, Hal B. ; Chanh, Tran C. ; Carey, Kenneth D. ; White, Gary L. ; Solomon, Alan ; Kennedy, Ronald C. / Serologic crossreactions among primate immunoglobulins. In: Developmental and Comparative Immunology. 1995 ; Vol. 19, No. 6. pp. 547-557.
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