Serologically defined V region subgroups of human λ light chains

Alan Solomon, D. T. Weiss

Research output: Contribution to journalArticle

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Abstract

The availability of numerous antisera prepared against λ-type Bence Jones proteins and λ chains of known amino acid sequence has led to the differentiation and classification of human λ light chains into one of five V(λ) subgroups. The five serologically defined subgroups V(λI), V(λII), V(λIII), V(λIV), and V(λVI), correspond to the chemical classification that is based on sequence homologies in the first framework region (FR1). Proteins designated by sequence as λV react with specific anti-λII antisera and are thus included in the V(λII) subgroup classification. The isotypic nature of the five Vλ subgroups was evidenced through analyses of λ-type light chains that were isolated from the IgG of normal individuals. Based on analyses of 116 Bence Jones proteins, the frequency of distribution of the λI, λII/V, λIII, λIV, and λVI proteins in the normal λ chain population is estimated to be 27%, 37%, 23%, 3%, and 10%, respectively. This distribution of V(λ) subgroups was comparable to that found among 82 monoclonal Igλ proteins. Considerable V(λ) intragroup antigenic heterogeneity was also apparent. At least two sub-subgroups were identified among each of the five major V(λ) subgroups, implying the existence of multiple genes in the human V(λ) genome. The V(λ) classification of 54 Igλ proteins obtained from patients with primary or multiple myeloma-associated amyloidosis substantiated the preferential association of λVI light chains with amyloidosis AL and the predominance of the normally rare V(λVI) subgroup in this disease.

Original languageEnglish (US)
Pages (from-to)824-830
Number of pages7
JournalJournal of Immunology
Volume139
Issue number3
StatePublished - Jan 1 1987

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Bence Jones Protein
Light
Amyloidosis
Immune Sera
Proteins
Human Genome
Sequence Homology
Multiple Myeloma
Amino Acid Sequence
Immunoglobulin G
Population
Genes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Serologically defined V region subgroups of human λ light chains. / Solomon, Alan; Weiss, D. T.

In: Journal of Immunology, Vol. 139, No. 3, 01.01.1987, p. 824-830.

Research output: Contribution to journalArticle

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abstract = "The availability of numerous antisera prepared against λ-type Bence Jones proteins and λ chains of known amino acid sequence has led to the differentiation and classification of human λ light chains into one of five V(λ) subgroups. The five serologically defined subgroups V(λI), V(λII), V(λIII), V(λIV), and V(λVI), correspond to the chemical classification that is based on sequence homologies in the first framework region (FR1). Proteins designated by sequence as λV react with specific anti-λII antisera and are thus included in the V(λII) subgroup classification. The isotypic nature of the five Vλ subgroups was evidenced through analyses of λ-type light chains that were isolated from the IgG of normal individuals. Based on analyses of 116 Bence Jones proteins, the frequency of distribution of the λI, λII/V, λIII, λIV, and λVI proteins in the normal λ chain population is estimated to be 27{\%}, 37{\%}, 23{\%}, 3{\%}, and 10{\%}, respectively. This distribution of V(λ) subgroups was comparable to that found among 82 monoclonal Igλ proteins. Considerable V(λ) intragroup antigenic heterogeneity was also apparent. At least two sub-subgroups were identified among each of the five major V(λ) subgroups, implying the existence of multiple genes in the human V(λ) genome. The V(λ) classification of 54 Igλ proteins obtained from patients with primary or multiple myeloma-associated amyloidosis substantiated the preferential association of λVI light chains with amyloidosis AL and the predominance of the normally rare V(λVI) subgroup in this disease.",
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AB - The availability of numerous antisera prepared against λ-type Bence Jones proteins and λ chains of known amino acid sequence has led to the differentiation and classification of human λ light chains into one of five V(λ) subgroups. The five serologically defined subgroups V(λI), V(λII), V(λIII), V(λIV), and V(λVI), correspond to the chemical classification that is based on sequence homologies in the first framework region (FR1). Proteins designated by sequence as λV react with specific anti-λII antisera and are thus included in the V(λII) subgroup classification. The isotypic nature of the five Vλ subgroups was evidenced through analyses of λ-type light chains that were isolated from the IgG of normal individuals. Based on analyses of 116 Bence Jones proteins, the frequency of distribution of the λI, λII/V, λIII, λIV, and λVI proteins in the normal λ chain population is estimated to be 27%, 37%, 23%, 3%, and 10%, respectively. This distribution of V(λ) subgroups was comparable to that found among 82 monoclonal Igλ proteins. Considerable V(λ) intragroup antigenic heterogeneity was also apparent. At least two sub-subgroups were identified among each of the five major V(λ) subgroups, implying the existence of multiple genes in the human V(λ) genome. The V(λ) classification of 54 Igλ proteins obtained from patients with primary or multiple myeloma-associated amyloidosis substantiated the preferential association of λVI light chains with amyloidosis AL and the predominance of the normally rare V(λVI) subgroup in this disease.

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