Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia

A. Mike Tummers, Deidra Mountain, J. William Mix, Stacy S. Kirkpatrick, David C. Cassada, Scott Stevens, Michael Freeman, Mitchell Goldman, Oscar Grandas

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: A primary component in the development of intimal hyperplasia (IH) in response to vascular injury is basement membrane remodeling. Matrix metalloproteinases (MMPs) play a major role in this process by degradation of basement membrane proteins, mainly collagen type IV. Vascular injury initiates an inflammatory cascade with the release of tumor necrosis factor-alpha (TNFα), interleukin-1beta (IL-1β), and C-reactive protein (CRP). We hypothesize serum levels of these elements may serve as biomarkers of the development of IH. Methods and Results: At baseline, 2, 7, 10, and 14 days post-balloon angioplasty of the carotid artery, rat tissue samples were stained with Masson trichrome elastin to examine IH. Intima:media ratios (I:M) increased significantly over time postinjury. Serum samples were collected at the time of tissue sampling, and levels of MMP-2, MMP-9, collagen type IV, TNFα, IL-1β, and CRP were assayed using sandwich enzyme-linked immunosorbent assay (ELISA). MMP-2 serum levels at 7, 10, and 14 days postinjury were significantly elevated compared with baseline. Other elements were not significantly elevated. Conclusion: Early and persistent elevation in the serum levels of MMP-2 may be a useful biomarker of basement membrane remodeling and the presence of IH.

Original languageEnglish (US)
Pages (from-to)9-13
Number of pages5
JournalJournal of Surgical Research
Volume160
Issue number1
DOIs
StatePublished - May 1 2010

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Tunica Intima
Matrix Metalloproteinase 2
Hyperplasia
Basement Membrane
Collagen Type IV
Vascular System Injuries
Serum
Interleukin-1beta
C-Reactive Protein
Tumor Necrosis Factor-alpha
Biomarkers
Balloon Angioplasty
Elastin
Matrix Metalloproteinase 9
Matrix Metalloproteinases
Carotid Arteries
Membrane Proteins
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia. / Tummers, A. Mike; Mountain, Deidra; Mix, J. William; Kirkpatrick, Stacy S.; Cassada, David C.; Stevens, Scott; Freeman, Michael; Goldman, Mitchell; Grandas, Oscar.

In: Journal of Surgical Research, Vol. 160, No. 1, 01.05.2010, p. 9-13.

Research output: Contribution to journalArticle

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abstract = "Background: A primary component in the development of intimal hyperplasia (IH) in response to vascular injury is basement membrane remodeling. Matrix metalloproteinases (MMPs) play a major role in this process by degradation of basement membrane proteins, mainly collagen type IV. Vascular injury initiates an inflammatory cascade with the release of tumor necrosis factor-alpha (TNFα), interleukin-1beta (IL-1β), and C-reactive protein (CRP). We hypothesize serum levels of these elements may serve as biomarkers of the development of IH. Methods and Results: At baseline, 2, 7, 10, and 14 days post-balloon angioplasty of the carotid artery, rat tissue samples were stained with Masson trichrome elastin to examine IH. Intima:media ratios (I:M) increased significantly over time postinjury. Serum samples were collected at the time of tissue sampling, and levels of MMP-2, MMP-9, collagen type IV, TNFα, IL-1β, and CRP were assayed using sandwich enzyme-linked immunosorbent assay (ELISA). MMP-2 serum levels at 7, 10, and 14 days postinjury were significantly elevated compared with baseline. Other elements were not significantly elevated. Conclusion: Early and persistent elevation in the serum levels of MMP-2 may be a useful biomarker of basement membrane remodeling and the presence of IH.",
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AU - Stevens, Scott

AU - Freeman, Michael

AU - Goldman, Mitchell

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