Serum opacity factor (SOF) of Streptococcus pyogenes evokes antibodies that opsonize homologous and heterologous sof-positive serotypes of group A streptococci

Harry Courtney, David L. Hasty, James Dale

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Serum opacity factor (SOF) is a protein expressed by Streptococcus pyogenes that opacifies mammalian serum. SOF is also a virulence factor of S. pyogenes, but it has not been previously shown to elicit a protective immune response. Herein, we report that SOF evokes bactericidal antibodies against S. pyogenes in humans, rabbits, and mice. Rabbit antiserum against purified recombinant SOF2 opsonized SOF-positive M type 2, 4, and 28 S. pyogenes in human blood but had no effect on SOF-negative M type 5 S. pyogenes. Furthermore, affinity-purified human antibodies against SOF2 also opsonized SOF-positive streptococci. A combination of antisera against M2 and SOF2 proteins was dramatically more effective in killing streptococci than either antiserum alone, indicating that antibodies against SOF2 enhance the opsonic efficiency of M protein antibodies. Mice tolerated an intravenous injection of 100 μg of SOF without overt signs of toxicity, and immunization with SOF protected mice against challenge infections with M type 2 S. pyogenes. These data indicate that SOF evokes opsonic antibodies that may protect against infections by SOF-positive serotypes of group A streptococci and suggest that different serotypes of SOF have common epitopes that may be useful vaccine candidates to protect against group A streptococcal infections.

Original languageEnglish (US)
Pages (from-to)5097-5103
Number of pages7
JournalInfection and Immunity
Volume71
Issue number9
DOIs
StatePublished - Sep 1 2003

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Streptococcus pyogenes
Streptococcus
Antibodies
Immune Sera
Serogroup
opacity factor
Rabbits
Streptococcal Infections
Proteins
Virulence Factors
Infection
Intravenous Injections
Epitopes
Immunization
Vaccines

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Serum opacity factor (SOF) of Streptococcus pyogenes evokes antibodies that opsonize homologous and heterologous sof-positive serotypes of group A streptococci. / Courtney, Harry; Hasty, David L.; Dale, James.

In: Infection and Immunity, Vol. 71, No. 9, 01.09.2003, p. 5097-5103.

Research output: Contribution to journalArticle

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