Shear stress alters pleural mesothelial cell permeability in culture

Christopher Waters, Matthew R. Glucksberg, Natacha Depaola, Julie Chang, James B. Grotberg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The sliding motion of the lung against the chest wall creates a shear stress in the pleural space, which can be as high as 60 dyn/cm2, depending on the respiration rate. Such shear stresses may affect the mesothelial cells that line the pleural space on the lung (visceral pleura) and chest wall (parietal pleura). When exposed to shear stress (17 dyn/cm2) in a parallel- plate flow chamber for 22 h, rat visceral pleura mesothelial cells were not altered morphologically and did not align in the direction of flow, in contrast to the shape changes observed for bovine aortic endothelial cells. By using mesothelial cells cultured on porous microcarrier beads, we measured the permeability of the cells at different flows in a cell-column chromatography assay. The permeabilities to sodium fluorescein and cyanocobalamin increased from 8.2 ± 1.0 and 7.8 ± 0.7 x 10-5 cm/s to 22.5 ± 1.2 and 21.8 ± 3.0 x 10-5 cm/s, respectively, when the flow was increased from 0.9 to 3.5 ml/min (corresponding to average shear stresses of 4.7-18.4 dyn/cm2). The permeabilities returned to baseline values when the flow was reduced. Cytochalasin D stimulated an increase in permeability that was not augmented by a subsequent increase in shear stress. These results suggest that the barrier function of mesothelial cells is responsive to changes in fluid shear stress.

Original languageEnglish (US)
Pages (from-to)448-458
Number of pages11
JournalJournal of Applied Physiology
Volume81
Issue number1
DOIs
StatePublished - Jan 1 1996

Fingerprint

Pleura
Permeability
Thoracic Wall
Cytochalasin D
Lung
Vitamin B 12
Respiratory Rate
Fluorescein
Chromatography
Cultured Cells
Endothelial Cells
Cell Line

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Waters, C., Glucksberg, M. R., Depaola, N., Chang, J., & Grotberg, J. B. (1996). Shear stress alters pleural mesothelial cell permeability in culture. Journal of Applied Physiology, 81(1), 448-458. https://doi.org/10.1152/jappl.1996.81.1.448

Shear stress alters pleural mesothelial cell permeability in culture. / Waters, Christopher; Glucksberg, Matthew R.; Depaola, Natacha; Chang, Julie; Grotberg, James B.

In: Journal of Applied Physiology, Vol. 81, No. 1, 01.01.1996, p. 448-458.

Research output: Contribution to journalArticle

Waters, C, Glucksberg, MR, Depaola, N, Chang, J & Grotberg, JB 1996, 'Shear stress alters pleural mesothelial cell permeability in culture', Journal of Applied Physiology, vol. 81, no. 1, pp. 448-458. https://doi.org/10.1152/jappl.1996.81.1.448
Waters, Christopher ; Glucksberg, Matthew R. ; Depaola, Natacha ; Chang, Julie ; Grotberg, James B. / Shear stress alters pleural mesothelial cell permeability in culture. In: Journal of Applied Physiology. 1996 ; Vol. 81, No. 1. pp. 448-458.
@article{1ee412d3b9f647cd8abf41ceceee6149,
title = "Shear stress alters pleural mesothelial cell permeability in culture",
abstract = "The sliding motion of the lung against the chest wall creates a shear stress in the pleural space, which can be as high as 60 dyn/cm2, depending on the respiration rate. Such shear stresses may affect the mesothelial cells that line the pleural space on the lung (visceral pleura) and chest wall (parietal pleura). When exposed to shear stress (17 dyn/cm2) in a parallel- plate flow chamber for 22 h, rat visceral pleura mesothelial cells were not altered morphologically and did not align in the direction of flow, in contrast to the shape changes observed for bovine aortic endothelial cells. By using mesothelial cells cultured on porous microcarrier beads, we measured the permeability of the cells at different flows in a cell-column chromatography assay. The permeabilities to sodium fluorescein and cyanocobalamin increased from 8.2 ± 1.0 and 7.8 ± 0.7 x 10-5 cm/s to 22.5 ± 1.2 and 21.8 ± 3.0 x 10-5 cm/s, respectively, when the flow was increased from 0.9 to 3.5 ml/min (corresponding to average shear stresses of 4.7-18.4 dyn/cm2). The permeabilities returned to baseline values when the flow was reduced. Cytochalasin D stimulated an increase in permeability that was not augmented by a subsequent increase in shear stress. These results suggest that the barrier function of mesothelial cells is responsive to changes in fluid shear stress.",
author = "Christopher Waters and Glucksberg, {Matthew R.} and Natacha Depaola and Julie Chang and Grotberg, {James B.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1152/jappl.1996.81.1.448",
language = "English (US)",
volume = "81",
pages = "448--458",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Shear stress alters pleural mesothelial cell permeability in culture

AU - Waters, Christopher

AU - Glucksberg, Matthew R.

AU - Depaola, Natacha

AU - Chang, Julie

AU - Grotberg, James B.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The sliding motion of the lung against the chest wall creates a shear stress in the pleural space, which can be as high as 60 dyn/cm2, depending on the respiration rate. Such shear stresses may affect the mesothelial cells that line the pleural space on the lung (visceral pleura) and chest wall (parietal pleura). When exposed to shear stress (17 dyn/cm2) in a parallel- plate flow chamber for 22 h, rat visceral pleura mesothelial cells were not altered morphologically and did not align in the direction of flow, in contrast to the shape changes observed for bovine aortic endothelial cells. By using mesothelial cells cultured on porous microcarrier beads, we measured the permeability of the cells at different flows in a cell-column chromatography assay. The permeabilities to sodium fluorescein and cyanocobalamin increased from 8.2 ± 1.0 and 7.8 ± 0.7 x 10-5 cm/s to 22.5 ± 1.2 and 21.8 ± 3.0 x 10-5 cm/s, respectively, when the flow was increased from 0.9 to 3.5 ml/min (corresponding to average shear stresses of 4.7-18.4 dyn/cm2). The permeabilities returned to baseline values when the flow was reduced. Cytochalasin D stimulated an increase in permeability that was not augmented by a subsequent increase in shear stress. These results suggest that the barrier function of mesothelial cells is responsive to changes in fluid shear stress.

AB - The sliding motion of the lung against the chest wall creates a shear stress in the pleural space, which can be as high as 60 dyn/cm2, depending on the respiration rate. Such shear stresses may affect the mesothelial cells that line the pleural space on the lung (visceral pleura) and chest wall (parietal pleura). When exposed to shear stress (17 dyn/cm2) in a parallel- plate flow chamber for 22 h, rat visceral pleura mesothelial cells were not altered morphologically and did not align in the direction of flow, in contrast to the shape changes observed for bovine aortic endothelial cells. By using mesothelial cells cultured on porous microcarrier beads, we measured the permeability of the cells at different flows in a cell-column chromatography assay. The permeabilities to sodium fluorescein and cyanocobalamin increased from 8.2 ± 1.0 and 7.8 ± 0.7 x 10-5 cm/s to 22.5 ± 1.2 and 21.8 ± 3.0 x 10-5 cm/s, respectively, when the flow was increased from 0.9 to 3.5 ml/min (corresponding to average shear stresses of 4.7-18.4 dyn/cm2). The permeabilities returned to baseline values when the flow was reduced. Cytochalasin D stimulated an increase in permeability that was not augmented by a subsequent increase in shear stress. These results suggest that the barrier function of mesothelial cells is responsive to changes in fluid shear stress.

UR - http://www.scopus.com/inward/record.url?scp=0030056502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030056502&partnerID=8YFLogxK

U2 - 10.1152/jappl.1996.81.1.448

DO - 10.1152/jappl.1996.81.1.448

M3 - Article

VL - 81

SP - 448

EP - 458

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 1

ER -