SHN-1, a Shank homologue in C. elegans, affects defecation rhythm via the inositol-1,4,5-trisphosphate receptor

Chang Hoon Jee, Jungsoo Lee, Jin Il Lee, Won Hae Lee, Byung Jae Park, Jae Ran Yu, Eunhye Park, Eunjoon Kim, Joohong Ahnn

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Protein localization in the postsynaptic density (PSD) of neurons is mediated by scaffolding proteins such as PSD-95 and Shank, which ensure proper function of receptors at the membrane. The Shank family of scaffolding proteins contain PDZ (PSD-95, Dlg, and ZO-1) domains and have been implicated in the localizations of many receptor proteins including glutamate receptors in mammals. We have identified and characterized shn-1, the only homologue of Shank in Caenorhabditis elegans. The shn-1 gene shows approximately 40% identity over 1000 amino acids to rat Shanks. SHN-1 protein is localized in various tissues including neurons, pharynx and intestine. RNAi suppression of SHN-1 did not cause lethality or developmental abnormality. However, suppression of SHN-1 in the itr-1 (sa73) mutant, which has a defective inositol-1,4,5-trisphosphate (IP3) receptor, resulted in animals with altered defecation rhythm. Our data suggest a possible role of SHN-1 in affecting function of IP3 receptors in C. elegans.

Original languageEnglish (US)
Pages (from-to)29-36
Number of pages8
JournalFEBS Letters
Volume561
Issue number1-3
DOIs
StatePublished - Mar 12 2004

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Inositol 1,4,5-Trisphosphate Receptors
Defecation
Post-Synaptic Density
Caenorhabditis elegans
Proteins
Neurons
Inositol 1,4,5-Trisphosphate
Glutamate Receptors
Pharynx
RNA Interference
Mammals
Intestines
Rats
Animals
Amino Acids
Genes
Membranes
Tissue

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

SHN-1, a Shank homologue in C. elegans, affects defecation rhythm via the inositol-1,4,5-trisphosphate receptor. / Jee, Chang Hoon; Lee, Jungsoo; Lee, Jin Il; Lee, Won Hae; Park, Byung Jae; Yu, Jae Ran; Park, Eunhye; Kim, Eunjoon; Ahnn, Joohong.

In: FEBS Letters, Vol. 561, No. 1-3, 12.03.2004, p. 29-36.

Research output: Contribution to journalArticle

Jee, Chang Hoon ; Lee, Jungsoo ; Lee, Jin Il ; Lee, Won Hae ; Park, Byung Jae ; Yu, Jae Ran ; Park, Eunhye ; Kim, Eunjoon ; Ahnn, Joohong. / SHN-1, a Shank homologue in C. elegans, affects defecation rhythm via the inositol-1,4,5-trisphosphate receptor. In: FEBS Letters. 2004 ; Vol. 561, No. 1-3. pp. 29-36.
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