Short-term effects of ankaferd hemostat for renal artery embolization

An experimental study

Orhan Ozbek, Kadir Acar, Osman Koc, Kadir Saritas, Hatice Toy, Yalcin Solak, Seda Ozbek, Ahmet Kucukapan, Ibrahim Guler, Abduzhappar Gaipov, Suleyman Turk, Ibrahim Celaleddin Haznedaroglu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: Renal artery embolization (RAE) is a minimally invasive therapeutic technique that is utilized in a number of disorders. Ankaferd is a novel hemostatic agent with a new mechanism of action independent of clotting factors. We used Ankaferd for RAE in a sheep model. Methods: Seven adult female sheep were included in the study. Selective renal arteriogram using 5-F diagnostic catheter was performed to make sure that each kidney was fed by a single renal artery and the animal had normal renal vasculature. Coaxial 2.7-F microcatheter was advanced to the distal main renal artery. Under fluoroscopic guidance, 2 mL of Ankaferd mixed with 2 mL of nonionic iodinated contrast agent was slowly injected. Fluoroscopy was used to observe the deceleration of flow and stagnation. Control renal angiograms were performed just after embolization. After the procedure, the animals were observed for 1 day and then sacrificed with intravenous sodium thiopental. Results: The technical success was observed in seven of the seven animals. After embolization procedure, none of the animals died or experienced a major systemic adverse event. On macroscopic examination of the embolized kidneys, thrombus at the level of main renal artery formed after Ankaferd embolization was more compact compared with the thrombi that was not Ankaferd-associated, which was observed elsewhere. Microscopically, majority of the renal tubular cells (80-90 %) were necrotic, and there was epithelial cell damage in a small portion of the cells (10-20 %). Conclusions: RAE was safe and effective in the short-term with Ankaferd in studied animals. Further studies should be conducted to better delineate the embolizing potential of this novel hemostatic agent.

Original languageEnglish (US)
Pages (from-to)498-504
Number of pages7
JournalCardiovascular and Interventional Radiology
Volume36
Issue number2
DOIs
StatePublished - Apr 1 2013

Fingerprint

Renal Artery
Kidney
Hemostatics
Sheep
Thrombosis
Blood Coagulation Factors
Deceleration
Thiopental
Fluoroscopy
Contrast Media
Angiography
Catheters
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

Short-term effects of ankaferd hemostat for renal artery embolization : An experimental study. / Ozbek, Orhan; Acar, Kadir; Koc, Osman; Saritas, Kadir; Toy, Hatice; Solak, Yalcin; Ozbek, Seda; Kucukapan, Ahmet; Guler, Ibrahim; Gaipov, Abduzhappar; Turk, Suleyman; Haznedaroglu, Ibrahim Celaleddin.

In: Cardiovascular and Interventional Radiology, Vol. 36, No. 2, 01.04.2013, p. 498-504.

Research output: Contribution to journalArticle

Ozbek, O, Acar, K, Koc, O, Saritas, K, Toy, H, Solak, Y, Ozbek, S, Kucukapan, A, Guler, I, Gaipov, A, Turk, S & Haznedaroglu, IC 2013, 'Short-term effects of ankaferd hemostat for renal artery embolization: An experimental study', Cardiovascular and Interventional Radiology, vol. 36, no. 2, pp. 498-504. https://doi.org/10.1007/s00270-012-0419-8
Ozbek, Orhan ; Acar, Kadir ; Koc, Osman ; Saritas, Kadir ; Toy, Hatice ; Solak, Yalcin ; Ozbek, Seda ; Kucukapan, Ahmet ; Guler, Ibrahim ; Gaipov, Abduzhappar ; Turk, Suleyman ; Haznedaroglu, Ibrahim Celaleddin. / Short-term effects of ankaferd hemostat for renal artery embolization : An experimental study. In: Cardiovascular and Interventional Radiology. 2013 ; Vol. 36, No. 2. pp. 498-504.
@article{aca9d0ec28ae4c3985770b6d4d1d8550,
title = "Short-term effects of ankaferd hemostat for renal artery embolization: An experimental study",
abstract = "Purpose: Renal artery embolization (RAE) is a minimally invasive therapeutic technique that is utilized in a number of disorders. Ankaferd is a novel hemostatic agent with a new mechanism of action independent of clotting factors. We used Ankaferd for RAE in a sheep model. Methods: Seven adult female sheep were included in the study. Selective renal arteriogram using 5-F diagnostic catheter was performed to make sure that each kidney was fed by a single renal artery and the animal had normal renal vasculature. Coaxial 2.7-F microcatheter was advanced to the distal main renal artery. Under fluoroscopic guidance, 2 mL of Ankaferd mixed with 2 mL of nonionic iodinated contrast agent was slowly injected. Fluoroscopy was used to observe the deceleration of flow and stagnation. Control renal angiograms were performed just after embolization. After the procedure, the animals were observed for 1 day and then sacrificed with intravenous sodium thiopental. Results: The technical success was observed in seven of the seven animals. After embolization procedure, none of the animals died or experienced a major systemic adverse event. On macroscopic examination of the embolized kidneys, thrombus at the level of main renal artery formed after Ankaferd embolization was more compact compared with the thrombi that was not Ankaferd-associated, which was observed elsewhere. Microscopically, majority of the renal tubular cells (80-90 {\%}) were necrotic, and there was epithelial cell damage in a small portion of the cells (10-20 {\%}). Conclusions: RAE was safe and effective in the short-term with Ankaferd in studied animals. Further studies should be conducted to better delineate the embolizing potential of this novel hemostatic agent.",
author = "Orhan Ozbek and Kadir Acar and Osman Koc and Kadir Saritas and Hatice Toy and Yalcin Solak and Seda Ozbek and Ahmet Kucukapan and Ibrahim Guler and Abduzhappar Gaipov and Suleyman Turk and Haznedaroglu, {Ibrahim Celaleddin}",
year = "2013",
month = "4",
day = "1",
doi = "10.1007/s00270-012-0419-8",
language = "English (US)",
volume = "36",
pages = "498--504",
journal = "CardioVascular and Interventional Radiology",
issn = "7415-5101",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Short-term effects of ankaferd hemostat for renal artery embolization

T2 - An experimental study

AU - Ozbek, Orhan

AU - Acar, Kadir

AU - Koc, Osman

AU - Saritas, Kadir

AU - Toy, Hatice

AU - Solak, Yalcin

AU - Ozbek, Seda

AU - Kucukapan, Ahmet

AU - Guler, Ibrahim

AU - Gaipov, Abduzhappar

AU - Turk, Suleyman

AU - Haznedaroglu, Ibrahim Celaleddin

PY - 2013/4/1

Y1 - 2013/4/1

N2 - Purpose: Renal artery embolization (RAE) is a minimally invasive therapeutic technique that is utilized in a number of disorders. Ankaferd is a novel hemostatic agent with a new mechanism of action independent of clotting factors. We used Ankaferd for RAE in a sheep model. Methods: Seven adult female sheep were included in the study. Selective renal arteriogram using 5-F diagnostic catheter was performed to make sure that each kidney was fed by a single renal artery and the animal had normal renal vasculature. Coaxial 2.7-F microcatheter was advanced to the distal main renal artery. Under fluoroscopic guidance, 2 mL of Ankaferd mixed with 2 mL of nonionic iodinated contrast agent was slowly injected. Fluoroscopy was used to observe the deceleration of flow and stagnation. Control renal angiograms were performed just after embolization. After the procedure, the animals were observed for 1 day and then sacrificed with intravenous sodium thiopental. Results: The technical success was observed in seven of the seven animals. After embolization procedure, none of the animals died or experienced a major systemic adverse event. On macroscopic examination of the embolized kidneys, thrombus at the level of main renal artery formed after Ankaferd embolization was more compact compared with the thrombi that was not Ankaferd-associated, which was observed elsewhere. Microscopically, majority of the renal tubular cells (80-90 %) were necrotic, and there was epithelial cell damage in a small portion of the cells (10-20 %). Conclusions: RAE was safe and effective in the short-term with Ankaferd in studied animals. Further studies should be conducted to better delineate the embolizing potential of this novel hemostatic agent.

AB - Purpose: Renal artery embolization (RAE) is a minimally invasive therapeutic technique that is utilized in a number of disorders. Ankaferd is a novel hemostatic agent with a new mechanism of action independent of clotting factors. We used Ankaferd for RAE in a sheep model. Methods: Seven adult female sheep were included in the study. Selective renal arteriogram using 5-F diagnostic catheter was performed to make sure that each kidney was fed by a single renal artery and the animal had normal renal vasculature. Coaxial 2.7-F microcatheter was advanced to the distal main renal artery. Under fluoroscopic guidance, 2 mL of Ankaferd mixed with 2 mL of nonionic iodinated contrast agent was slowly injected. Fluoroscopy was used to observe the deceleration of flow and stagnation. Control renal angiograms were performed just after embolization. After the procedure, the animals were observed for 1 day and then sacrificed with intravenous sodium thiopental. Results: The technical success was observed in seven of the seven animals. After embolization procedure, none of the animals died or experienced a major systemic adverse event. On macroscopic examination of the embolized kidneys, thrombus at the level of main renal artery formed after Ankaferd embolization was more compact compared with the thrombi that was not Ankaferd-associated, which was observed elsewhere. Microscopically, majority of the renal tubular cells (80-90 %) were necrotic, and there was epithelial cell damage in a small portion of the cells (10-20 %). Conclusions: RAE was safe and effective in the short-term with Ankaferd in studied animals. Further studies should be conducted to better delineate the embolizing potential of this novel hemostatic agent.

UR - http://www.scopus.com/inward/record.url?scp=84875225770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875225770&partnerID=8YFLogxK

U2 - 10.1007/s00270-012-0419-8

DO - 10.1007/s00270-012-0419-8

M3 - Article

VL - 36

SP - 498

EP - 504

JO - CardioVascular and Interventional Radiology

JF - CardioVascular and Interventional Radiology

SN - 7415-5101

IS - 2

ER -