Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin

Manuel Toledano, Fátima S. Aguilera, Inmaculada Cabello, Manuel Toledano-Osorio, Estrella Osorio, Modesto T. López-López, Franklin Garcia-Godoy, Christopher D. Lynch, Raquel Osorio

Research output: Contribution to journalArticle

Abstract

Background: The aim was to evaluate the effect of silver loaded nanoparticles (NPs) application on the triboscopic, crystallographic and viscoelastic properties of demineralized dentin. Polymethylmetacrylate-based NPs and Ag loaded NPs were applied on demineralized dentin. Material and Methods: Treated and untreated surfaces were probed by a nanoindenter to test viscoelasticity, and by atomic force microscopy to test nanoroughness and collagen fibril diameter. X-ray diffraction and transmission electron microscopy through selected area diffraction and bright-field imaging were also used. Results: Dentin treated with Ag-NPs attained the lowest complex modulus, and the highest tan delta values after 7 days of storage. Dentin treated with undoped-NPs achieved the lowest nanoroughness and the greatest collagen bandwidths among groups. Crystals were identified as hydroxyapatite with the highest crystallographic maturity and crystallite size in dentin treated with undoped-NPs. Texture increased in all samples from 24 h to 7 d, except in dentin surfaces treated with Ag-NPs at 310 plane. Polyhedral, block-like, hexagonal or plate-like shaped apatite crystals constituted the bulk of minerals in dentin treated with Ag-NPs, after 7 d. Polyhedral or rounded/drop-like, and polymorphic in strata crystal apatite characterized the minerals when undoped-NPs were used, with more crystalline characteristics after 7 d than that found when Ag-NPs were applied. Ag-NPs application did not improve the mechanical performance of dentin and did not produce dentin remineralization. However, energy was dissipated through the dentin without showing stress concentration; contrary was occurring at dentin treated with undoped- NPs, that provoked bridge-like mineral deposits at the dentin surface. Conclusions: Ag-NPs application did not enhance the mechanical properties of cervical dentin, though the energy dissipation did not damage the dentin structure. Remineralization at dentin was not produced after Ag-NPs application, though improved crystallinity may lead to increase stability of the apatite that was generated at the dentin surface.

Original languageEnglish (US)
Pages (from-to)e156-e164
JournalMedicina Oral Patologia Oral y Cirugia Bucal
Volume24
Issue number2
DOIs
StatePublished - Mar 1 2019

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Dentin
Silver
Nanoparticles
Apatites
Minerals
Collagen
Atomic Force Microscopy
Polymethyl Methacrylate
Durapatite
Transmission Electron Microscopy
X-Ray Diffraction

All Science Journal Classification (ASJC) codes

  • Surgery
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Toledano, M., Aguilera, F. S., Cabello, I., Toledano-Osorio, M., Osorio, E., López-López, M. T., ... Osorio, R. (2019). Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin. Medicina Oral Patologia Oral y Cirugia Bucal, 24(2), e156-e164. https://doi.org/10.4317/medoral.22885

Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin. / Toledano, Manuel; Aguilera, Fátima S.; Cabello, Inmaculada; Toledano-Osorio, Manuel; Osorio, Estrella; López-López, Modesto T.; Garcia-Godoy, Franklin; Lynch, Christopher D.; Osorio, Raquel.

In: Medicina Oral Patologia Oral y Cirugia Bucal, Vol. 24, No. 2, 01.03.2019, p. e156-e164.

Research output: Contribution to journalArticle

Toledano, M, Aguilera, FS, Cabello, I, Toledano-Osorio, M, Osorio, E, López-López, MT, Garcia-Godoy, F, Lynch, CD & Osorio, R 2019, 'Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin', Medicina Oral Patologia Oral y Cirugia Bucal, vol. 24, no. 2, pp. e156-e164. https://doi.org/10.4317/medoral.22885
Toledano M, Aguilera FS, Cabello I, Toledano-Osorio M, Osorio E, López-López MT et al. Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin. Medicina Oral Patologia Oral y Cirugia Bucal. 2019 Mar 1;24(2):e156-e164. https://doi.org/10.4317/medoral.22885
Toledano, Manuel ; Aguilera, Fátima S. ; Cabello, Inmaculada ; Toledano-Osorio, Manuel ; Osorio, Estrella ; López-López, Modesto T. ; Garcia-Godoy, Franklin ; Lynch, Christopher D. ; Osorio, Raquel. / Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin. In: Medicina Oral Patologia Oral y Cirugia Bucal. 2019 ; Vol. 24, No. 2. pp. e156-e164.
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AU - Osorio, Estrella

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N2 - Background: The aim was to evaluate the effect of silver loaded nanoparticles (NPs) application on the triboscopic, crystallographic and viscoelastic properties of demineralized dentin. Polymethylmetacrylate-based NPs and Ag loaded NPs were applied on demineralized dentin. Material and Methods: Treated and untreated surfaces were probed by a nanoindenter to test viscoelasticity, and by atomic force microscopy to test nanoroughness and collagen fibril diameter. X-ray diffraction and transmission electron microscopy through selected area diffraction and bright-field imaging were also used. Results: Dentin treated with Ag-NPs attained the lowest complex modulus, and the highest tan delta values after 7 days of storage. Dentin treated with undoped-NPs achieved the lowest nanoroughness and the greatest collagen bandwidths among groups. Crystals were identified as hydroxyapatite with the highest crystallographic maturity and crystallite size in dentin treated with undoped-NPs. Texture increased in all samples from 24 h to 7 d, except in dentin surfaces treated with Ag-NPs at 310 plane. Polyhedral, block-like, hexagonal or plate-like shaped apatite crystals constituted the bulk of minerals in dentin treated with Ag-NPs, after 7 d. Polyhedral or rounded/drop-like, and polymorphic in strata crystal apatite characterized the minerals when undoped-NPs were used, with more crystalline characteristics after 7 d than that found when Ag-NPs were applied. Ag-NPs application did not improve the mechanical performance of dentin and did not produce dentin remineralization. However, energy was dissipated through the dentin without showing stress concentration; contrary was occurring at dentin treated with undoped- NPs, that provoked bridge-like mineral deposits at the dentin surface. Conclusions: Ag-NPs application did not enhance the mechanical properties of cervical dentin, though the energy dissipation did not damage the dentin structure. Remineralization at dentin was not produced after Ag-NPs application, though improved crystallinity may lead to increase stability of the apatite that was generated at the dentin surface.

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