Simultaneous assay of multiple antibiotics in human plasma by LC-MS/MS

Importance of optimizing formic acid concentration

Feng Chen, Zhe Yi Hu, Steven Laizure, Joanna Laizure

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aim: Optimal dosing of antibiotics in critically ill patients is complicated by the development of resistant organisms requiring treatment with multiple antibiotics and alterations in systemic exposure due to diseases and extracorporeal drug removal. Developing guidelines for optimal antibiotic dosing is an important therapeutic goal requiring robust analytical methods to simultaneously measure multiple antibiotics. Methods: An LC-MS/MS assay using protein precipitation for cleanup followed by a 6-min gradient separation was developed to simultaneously determine five antibiotics in human plasma. Results: The precision and accuracy were within the 15% acceptance range. The formic acid concentration was an important determinant of signal intensity, peak shape and matrix effects. Conclusion: The method was designed to be simple and successfully applied to a clinical pharmacokinetic study.

Original languageEnglish (US)
Pages (from-to)469-483
Number of pages15
JournalBioanalysis
Volume9
Issue number5
DOIs
StatePublished - Mar 1 2017

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formic acid
Plasma (human)
Assays
Anti-Bacterial Agents
Pharmacokinetics
Critical Illness
Guidelines

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

Simultaneous assay of multiple antibiotics in human plasma by LC-MS/MS : Importance of optimizing formic acid concentration. / Chen, Feng; Hu, Zhe Yi; Laizure, Steven; Laizure, Joanna.

In: Bioanalysis, Vol. 9, No. 5, 01.03.2017, p. 469-483.

Research output: Contribution to journalArticle

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