Simultaneous determination of calcium and magnesium in human blood serum by atomic absorption spectrophotometer

Syamal Bhattacharya, Edward Mallinckrodt

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Simultaneous determination of calcium and magnesium content in human blood serum has been reported as a routine clinical laboratory method, which is rapid, simple, and requires 50 X of serum for complete analysis with triplicate runs. This is accomplished using a Model 1250 Varian Techtron Atomic Absorption Spectrophotometer with the stoichiometric nitrous oxide-acetylene flame of 0.2 cm red-cone height. Calcium and magnesium standards and also the serum specimens are prepared in aqueous medium containing 2000 ppm potassium chloride. Serum specimens are ultimately diluted to 1:100 fold. About 40–50 samples can be analyzed per hour of instrument operation, if standard solutions and diluted serum specimens are readily available.

Original languageEnglish (US)
Pages (from-to)817-830
Number of pages14
JournalAnalytical Letters
Volume10
Issue number11
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

Fingerprint

Spectrophotometers
Magnesium
Calcium
Blood
Clinical laboratories
Acetylene
Potassium Chloride
Nitrous Oxide
Serum
Potassium
Cones
Oxides

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

Cite this

Simultaneous determination of calcium and magnesium in human blood serum by atomic absorption spectrophotometer. / Bhattacharya, Syamal; Mallinckrodt, Edward.

In: Analytical Letters, Vol. 10, No. 11, 01.01.1977, p. 817-830.

Research output: Contribution to journalArticle

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