Simultaneous quantification of seventeen bioactive components in rhizome and aerial parts of Alpinia officinarum Hance using LC-MS/MS

Jun Qing Zhang, Yong Wang, Hai Long Li, Qi Wen, Hang Yin, Nian Kai Zeng, Wei Yong Lai, Na Wei, Shou Qian Cheng, Sheng Li Kang, Feng Chen, You Bin Li

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The rhizomes of Alpinia officinarum Hance (Zingiberaceae family) have been used as antiemetics, stomachics and analgesics in Asia for centuries. Unfortunately, the aerial parts were thrown away as waste whilst harvesting the rhizomes of A. officinarum. Recently, scientists reported that the ethanol extract of the aerial parts displayed anti-proliferation activity through mitochondrial pathway-induced cell apoptosis. However, the chemical composition of this extract remained largely unknown. We have identified sixteen chemicals including twelve flavonoids and four diarylheptanoids from the methanol extract of A. officinarum leaves using liquid chromatography/tandem mass spectrometry (LC-MS/MS). In order to better explore the potential value of the aerial parts, we need to know what the main constituents occurring in the aerial parts are and how the content of these chemicals is influenced by growing period. In the present study, a LC-MS/MS method was developed and validated for determination of seventeen compounds occurring both in the aerial parts and rhizomes sampled at different growing periods. The validation indices evaluated were satisfactory and the method was successfully employed to analyze the above-mentioned plant samples. Notably, we found that the content of these compounds, except for quercetin, was higher in rhizomes than in aerial parts. The six major constituents both in the aerial parts and rhizomes were galangin, kaempferide, hexahydrocurcumin, pinocembrin, chrysin and isorhamnetin. Moreover, the trends of change in content along with the sample period for most of the monitored phytochemicals were almost similar between the aerial parts and rhizomes. Our study should be of value in arousing everyone's interest to make the best use of the aerial parts of A. officinarum in the future.

Original languageEnglish (US)
Pages (from-to)4919-4926
Number of pages8
JournalAnalytical Methods
Volume7
Issue number12
DOIs
StatePublished - Jan 1 2015

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Antennas
Diarylheptanoids
Flavonoids
Antiemetics
Quercetin
Liquid chromatography
Phytochemicals
Cell death
Mass spectrometry
Methanol
Analgesics
Ethanol
Apoptosis
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

Cite this

Simultaneous quantification of seventeen bioactive components in rhizome and aerial parts of Alpinia officinarum Hance using LC-MS/MS. / Zhang, Jun Qing; Wang, Yong; Li, Hai Long; Wen, Qi; Yin, Hang; Zeng, Nian Kai; Lai, Wei Yong; Wei, Na; Cheng, Shou Qian; Kang, Sheng Li; Chen, Feng; Li, You Bin.

In: Analytical Methods, Vol. 7, No. 12, 01.01.2015, p. 4919-4926.

Research output: Contribution to journalArticle

Zhang, JQ, Wang, Y, Li, HL, Wen, Q, Yin, H, Zeng, NK, Lai, WY, Wei, N, Cheng, SQ, Kang, SL, Chen, F & Li, YB 2015, 'Simultaneous quantification of seventeen bioactive components in rhizome and aerial parts of Alpinia officinarum Hance using LC-MS/MS', Analytical Methods, vol. 7, no. 12, pp. 4919-4926. https://doi.org/10.1039/c5ay00647c
Zhang, Jun Qing ; Wang, Yong ; Li, Hai Long ; Wen, Qi ; Yin, Hang ; Zeng, Nian Kai ; Lai, Wei Yong ; Wei, Na ; Cheng, Shou Qian ; Kang, Sheng Li ; Chen, Feng ; Li, You Bin. / Simultaneous quantification of seventeen bioactive components in rhizome and aerial parts of Alpinia officinarum Hance using LC-MS/MS. In: Analytical Methods. 2015 ; Vol. 7, No. 12. pp. 4919-4926.
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