Skeletal muscle mass recovery from atrophy in IL-6 knockout mice

T. A. Washington, J. P. White, J. M. Davis, L. B. Wilson, L. L. Lowe, S. Sato, James Carson

Research output: Contribution to journalArticle

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Abstract

Aim: Skeletal muscle interleukin-6 (IL-6) expression is induced by continuous contraction, overload-induced hypertrophy and during muscle regeneration. The loss of IL-6 can alter skeletal muscle's growth and extracellular matrix remodelling response to overload-induced hypertrophy. Insulin-like growth factor-1 (IGF-1) gene expression and related signalling through Akt/mTOR is a critical regulator of muscle mass. The significance of IL-6 expression during the recovery from muscle atrophy is unclear. This study's purpose was to determine the effect of IL-6 loss on mouse gastrocnemius (GAS) muscle mass during recovery from hindlimb suspension (HS)-induced atrophy. Methods: Female C57BL/6 [wild type (WT)] and IL-6 knockout (IL-6 KO) mice at 10weeks of age were assigned to control, HS or HS followed by normal cage ambulation groups. Results: GAS muscle atrophy was induced by 10days of HS. HS induced a 20% loss of GAS mass in both WT and IL-6 KO mice. HS+7days of recovery restored WT GAS mass to cage-control values. GAS mass from IL-6 KO mice did not return to cage-control values until HS+14days of recovery. Both IGF-1 mRNA expression and Akt/mTOR signalling were increased in WT muscle after 1day of recovery. In IL-6 KO muscle, IGF-1 mRNA expression was decreased and Akt/mTOR signalling was not induced after 1day of recovery. MyoD and myogenin mRNA expression were both induced in WT muscle after 1day of recovery, but not in IL-6 KO muscle. Conclusion: Muscle IL-6 expression appears important for the initial growth response during the recovery from disuse.

Original languageEnglish (US)
Pages (from-to)657-669
Number of pages13
JournalActa Physiologica
Volume202
Issue number4
DOIs
StatePublished - Aug 1 2011

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Hindlimb Suspension
Knockout Mice
Atrophy
Interleukin-6
Skeletal Muscle
Muscles
Somatomedins
Muscular Atrophy
Messenger RNA
Hypertrophy
Myogenin
Growth
Walking
Extracellular Matrix
Regeneration
Gene Expression

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Washington, T. A., White, J. P., Davis, J. M., Wilson, L. B., Lowe, L. L., Sato, S., & Carson, J. (2011). Skeletal muscle mass recovery from atrophy in IL-6 knockout mice. Acta Physiologica, 202(4), 657-669. https://doi.org/10.1111/j.1748-1716.2011.02281.x

Skeletal muscle mass recovery from atrophy in IL-6 knockout mice. / Washington, T. A.; White, J. P.; Davis, J. M.; Wilson, L. B.; Lowe, L. L.; Sato, S.; Carson, James.

In: Acta Physiologica, Vol. 202, No. 4, 01.08.2011, p. 657-669.

Research output: Contribution to journalArticle

Washington, TA, White, JP, Davis, JM, Wilson, LB, Lowe, LL, Sato, S & Carson, J 2011, 'Skeletal muscle mass recovery from atrophy in IL-6 knockout mice', Acta Physiologica, vol. 202, no. 4, pp. 657-669. https://doi.org/10.1111/j.1748-1716.2011.02281.x
Washington TA, White JP, Davis JM, Wilson LB, Lowe LL, Sato S et al. Skeletal muscle mass recovery from atrophy in IL-6 knockout mice. Acta Physiologica. 2011 Aug 1;202(4):657-669. https://doi.org/10.1111/j.1748-1716.2011.02281.x
Washington, T. A. ; White, J. P. ; Davis, J. M. ; Wilson, L. B. ; Lowe, L. L. ; Sato, S. ; Carson, James. / Skeletal muscle mass recovery from atrophy in IL-6 knockout mice. In: Acta Physiologica. 2011 ; Vol. 202, No. 4. pp. 657-669.
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AU - Davis, J. M.

AU - Wilson, L. B.

AU - Lowe, L. L.

AU - Sato, S.

AU - Carson, James

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