Slitlamp biomicroscopy and photographic image analysis of herpes simplex virus stromal keratitis

R. Wilhelmus, Bradley M. Mitchell, Chandler R. Dawson, Dan B. Jones, Bruce A. Barron, Herbert E. Kaufman, Joel Sugar, Robert A. Hyndiuk, Peter R. Laibson, R. Doyle Stulting, Penny Asbell

Research output: Contribution to journalArticle

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Abstract

Objective: To validate photographic bioimaging for evaluating the severity of herpes simplex virus keratitis. Methods: Stromal keratitis of patients in the Herpetic Eye Disease Study was clinically measured with a slitbeam micrometer and then photographed at trial entry. Calibrated images of 169 eyes were analyzed for the size, location, and density of stromal keratitis and endotheliitis, with shape factor as a function of area and perimeter. Validity was assessed by comparing clinical and computerized measurements and by correlating the keratitis area with visual acuity. Logistic regression explored characteristics associated with larger or denser corneal inflammation. Results: Stromal keratitis had a median area of 22.4 mm 2 (interquartile range, 12.8-31.6 mm 2) with amedian shape factor of 0.69 (interquartile range, 0.56-0.79); 126 eyes (75%) had their midpoint within 2 mm of the cornea's geometric center. Photoanalytical area estimates of herpetic stro-mal keratitis correlated closely with clinical measurements (correlation coefficient, 0.83). Eyes with larger stromal keratitis had worse vision (correlation coefficient, 0.32) and were more likely to have iritis (P=.01). Necrotizing stromal keratitis was significantly whiter (P=.02). Conclusions: Image analysis validly assesses the disci-form geometry of herpetic stromal keratitis and confirms that increased severity is associated with uveitis and reduced vision.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalArchives of Ophthalmology
Volume127
Issue number2
DOIs
StatePublished - Feb 1 2009

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Keratitis
Simplexvirus
Herpetic Keratitis
Iritis
Low Vision
Eye Diseases
Uveitis
Cornea
Visual Acuity
Logistic Models
Inflammation

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Slitlamp biomicroscopy and photographic image analysis of herpes simplex virus stromal keratitis. / Wilhelmus, R.; Mitchell, Bradley M.; Dawson, Chandler R.; Jones, Dan B.; Barron, Bruce A.; Kaufman, Herbert E.; Sugar, Joel; Hyndiuk, Robert A.; Laibson, Peter R.; Stulting, R. Doyle; Asbell, Penny.

In: Archives of Ophthalmology, Vol. 127, No. 2, 01.02.2009, p. 161-166.

Research output: Contribution to journalArticle

Wilhelmus, R, Mitchell, BM, Dawson, CR, Jones, DB, Barron, BA, Kaufman, HE, Sugar, J, Hyndiuk, RA, Laibson, PR, Stulting, RD & Asbell, P 2009, 'Slitlamp biomicroscopy and photographic image analysis of herpes simplex virus stromal keratitis', Archives of Ophthalmology, vol. 127, no. 2, pp. 161-166. https://doi.org/10.1001/archophthalmol.2008.577
Wilhelmus, R. ; Mitchell, Bradley M. ; Dawson, Chandler R. ; Jones, Dan B. ; Barron, Bruce A. ; Kaufman, Herbert E. ; Sugar, Joel ; Hyndiuk, Robert A. ; Laibson, Peter R. ; Stulting, R. Doyle ; Asbell, Penny. / Slitlamp biomicroscopy and photographic image analysis of herpes simplex virus stromal keratitis. In: Archives of Ophthalmology. 2009 ; Vol. 127, No. 2. pp. 161-166.
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