Small molecules affect human dental pulp stem cell properties via multiple signaling pathways

Mey Al-Habib, Zongdong Yu, George Huang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

One fundamental issue regarding stem cells for regenerative medicine is the maintenance of stem cell stemness. The purpose of the study was to test whether small molecules can enhance stem cell properties of mesenchymal stem cells (MSCs) derived from human dental pulp (hDPSCs), which have potential for multiple clinical applications. We identified the effects of small molecules (Pluripotin (SC1), 6-bromoindirubin-3-oxime and rapamycin) on the maintenance of hDPSC properties in vitro and the mechanisms involved in exerting the effects. Primary cultures of hDPSCs were exposed to optimal concentrations of these small molecules. Treated hDPSCs were analyzed for their proliferation, the expression levels of pluripotent and MSC markers, differentiation capacities, and intracellular signaling activations. We found that small molecule treatments decreased cell proliferation and increased the expression of STRO-1, NANOG, OCT4, and SOX2, while diminishing cell differentiation into odonto/osteogenic, adipogenic, and neurogenic lineages in vitro. These effects involved Ras-GAP-, ERK1/2-, and mTOR-signaling pathways, which may preserve the cell self-renewal capacity, while suppressing differentiation. We conclude that small molecules appear to enhance the immature state of hDPSCs in culture, which may be used as a strategy for adult stem cell maintenance and extend their capacity for regenerative applications.

Original languageEnglish (US)
Pages (from-to)2402-2413
Number of pages12
JournalStem Cells and Development
Volume22
Issue number17
DOIs
StatePublished - Sep 1 2013

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Dental Pulp
Stem Cells
Maintenance
Mesenchymal Stromal Cells
Cell Differentiation
ras GTPase-Activating Proteins
Pluripotent Stem Cells
Adult Stem Cells
Regenerative Medicine
Sirolimus
Cell Proliferation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Small molecules affect human dental pulp stem cell properties via multiple signaling pathways. / Al-Habib, Mey; Yu, Zongdong; Huang, George.

In: Stem Cells and Development, Vol. 22, No. 17, 01.09.2013, p. 2402-2413.

Research output: Contribution to journalArticle

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