Smear layer removal and chelated calcium ion quantification of three irrigating solutions

Andre Augusto Franco Marques, Melissa Marchesan, Celso Bernardo de Sousa-Filho, Yara Teresinha Correa Silva-Sousa, Manoel D. Sousa-Neto, Antonio Miranda da Cruz-Filho

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The purpose of this study was to evaluate, by scanning electron microscopy (SEM), smear layer removal and quantify, by atomic absorption spectrophotometry, the amount of calcium ion present in the chelating solutions after their use. Sixteen extracted canines were instrumented using the step-back technique and were assigned to 3 groups according to the irrigating solution used: G1: 1 mL 17% EDTAC between each file; G2: 1 mL 17% CDTA; G3: 1 mL 17% EGTA. The solutions were collected after use. The teeth were cleaved longitudinally, evaluated under SEM and assessed for smear layer by blinded examiners and scored from 1 to 4. In order to quantify calcium ion release, the collected solutions were examined by atomic absorption spectrophotometry. Freidman's test was used for statistical analysis of SEM values and showed that canals irrigated with 17% EDTAC and 17% CDTA had significantly less smear layer throughout the canals than 17% EGTA (p<0.01). For analysis of the collected solutions, Tukey's test was used and showed that EDTAC and CDTA had a greater amount of calcium ions (22.8±7.54 and 60.6±20.67 μg/mL, respectively) compared to EGTA (70.5±14.2 μg/mL) (p<0.01). The association both methodologies may contribute to the understanding of how these solutions act in the root canal.

Original languageEnglish (US)
Pages (from-to)306-309
Number of pages4
JournalBrazilian Dental Journal
Volume17
Issue number4
StatePublished - Dec 1 2006
Externally publishedYes

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Smear Layer
Ions
Calcium
Egtazic Acid
Electron Scanning Microscopy
Atomic Spectrophotometry
Dental Pulp Cavity
Canidae
Tooth
EDTAC
CDTA

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Marques, A. A. F., Marchesan, M., de Sousa-Filho, C. B., Silva-Sousa, Y. T. C., Sousa-Neto, M. D., & da Cruz-Filho, A. M. (2006). Smear layer removal and chelated calcium ion quantification of three irrigating solutions. Brazilian Dental Journal, 17(4), 306-309.

Smear layer removal and chelated calcium ion quantification of three irrigating solutions. / Marques, Andre Augusto Franco; Marchesan, Melissa; de Sousa-Filho, Celso Bernardo; Silva-Sousa, Yara Teresinha Correa; Sousa-Neto, Manoel D.; da Cruz-Filho, Antonio Miranda.

In: Brazilian Dental Journal, Vol. 17, No. 4, 01.12.2006, p. 306-309.

Research output: Contribution to journalArticle

Marques, AAF, Marchesan, M, de Sousa-Filho, CB, Silva-Sousa, YTC, Sousa-Neto, MD & da Cruz-Filho, AM 2006, 'Smear layer removal and chelated calcium ion quantification of three irrigating solutions', Brazilian Dental Journal, vol. 17, no. 4, pp. 306-309.
Marques AAF, Marchesan M, de Sousa-Filho CB, Silva-Sousa YTC, Sousa-Neto MD, da Cruz-Filho AM. Smear layer removal and chelated calcium ion quantification of three irrigating solutions. Brazilian Dental Journal. 2006 Dec 1;17(4):306-309.
Marques, Andre Augusto Franco ; Marchesan, Melissa ; de Sousa-Filho, Celso Bernardo ; Silva-Sousa, Yara Teresinha Correa ; Sousa-Neto, Manoel D. ; da Cruz-Filho, Antonio Miranda. / Smear layer removal and chelated calcium ion quantification of three irrigating solutions. In: Brazilian Dental Journal. 2006 ; Vol. 17, No. 4. pp. 306-309.
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