Solid-phase radioimmunoassay of fragment D of human fibrinogen by use of a low-affinity monoclonal antibody

Stephen Kennel, P. K. Lankford

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Increased concentrations of plasmin-catalyzed degradation products of fibrin and fibrinogen in body fluids have been correlated with many disease states. We have developed monoclonal antibodies that react with one of these products, fragment D. Antibodies binding fragment D but not fibrinogen were found to have low affinity constants (K(a) = 107 L/mol). A double monoclonal-antibody assay was developed in which a specific antibody attached to Sepharose beads is used to concentrate fragment D selectively from a test sample. Fragment D bound to the washed beads was then quantified with a high affinity (K(a) = 1010 L/mol) monoclonal antibody that reacts equally well with fragment D and fibrinogen. This solid-phase assay is shown to be specific and about 10-fold more sensitive than a standard liquid-phase competition radioimmunoassay in which the same antibody was used.

Original languageEnglish (US)
Pages (from-to)778-781
Number of pages4
JournalClinical Chemistry
Volume29
Issue number5
StatePublished - 1983
Externally publishedYes

Fingerprint

Fibrinogen
Radioimmunoassay
Monoclonal Antibodies
Antibodies
Assays
Fibrin Fibrinogen Degradation Products
Immunoglobulin Fragments
Fibrinolysin
Body fluids
Body Fluids
Sepharose
Liquids
fibrinogen D fragment

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Solid-phase radioimmunoassay of fragment D of human fibrinogen by use of a low-affinity monoclonal antibody. / Kennel, Stephen; Lankford, P. K.

In: Clinical Chemistry, Vol. 29, No. 5, 1983, p. 778-781.

Research output: Contribution to journalArticle

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