Soluble domain 1 of platelet-endothelial cell adhesion molecule (PECAM) is sufficient to block transendothelial migration in vitro and in vivo

Francesca-Fang Liao, Jahanara Ali, Tricia Greene, William A. Muller

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

The inflammatory response involves sequential adhesive interactions between cell adhesion molecules of leukocytes and the endothelium. Unlike the several adhesive steps that precede it, transendothelial migration (diapedesis), the step in which leukocytes migrate between apposed endothelial cells, appears to involve primarily one adhesion molecule, platelet-endothelial cell adhesion molecule (PECAM, CD31). Therefore, we have focused on PECAM as a target for antiinflammatory therapy. We demonstrate that soluble chimeras made of the entire extracellular portion of PECAM, or of only the first immunoglobulin domain of PECAM, fused to the Fc portion of IgG, block diapedesis in vitro and in vivo. Furthermore, the truncated form of the PECAM-IgG chimera does not bind stably to its cellular ligand. This raises the possibility of selective anti-PECAM therapies that would not have the untoward opsonic or cell-activating properties of antibodies directed against PECAM.

Original languageEnglish (US)
Pages (from-to)1349-1357
Number of pages9
JournalJournal of Experimental Medicine
Volume185
Issue number7
DOIs
StatePublished - Apr 7 1997

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CD31 Antigens
Transendothelial and Transepithelial Migration
Cell Adhesion Molecules
Endothelial Cells
Blood Platelets
Adhesives
Leukocytes
Immunoglobulin G
In Vitro Techniques
Endothelium
Anti-Inflammatory Agents
Ligands

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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Soluble domain 1 of platelet-endothelial cell adhesion molecule (PECAM) is sufficient to block transendothelial migration in vitro and in vivo. / Liao, Francesca-Fang; Ali, Jahanara; Greene, Tricia; Muller, William A.

In: Journal of Experimental Medicine, Vol. 185, No. 7, 07.04.1997, p. 1349-1357.

Research output: Contribution to journalArticle

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