Soluble recombinant human metapneumovirus G protein is immunogenic but not protective

Alex Ryder, Sharon J. Tollefson, Amy B. Podsiad, Joyce E. Johnson, John V. Williams

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Human metapneumovirus (HMPV) expresses the major surface glycoproteins F and G. We evaluated the protective efficacy of immunization with G. We generated a recombinant form of G ectodomain (GΔTM) that was secreted from mammalian cells and purified by affinity chromatography. We tested the immunogenicity of GΔTM in cotton rats. Animals were immunized with PBS, GΔTM alone or adjuvanted, or were infected once with HMPV, and challenged with live HMPV at 28 days. Animals vaccinated with adjuvanted and non-adjuvanted GΔTM developed high levels of serum antibodies to both recombinant and native G protein; however, vaccinated animals did not develop neutralizing antibodies and were not protected against virus challenge. Unlike the analogous non-fusion glycoproteins of other human paramyxoviruses, HMPV G does not appear to be a protective antigen. This represents an unusual feature of HMPV.

Original languageEnglish (US)
Pages (from-to)4145-4152
Number of pages8
JournalVaccine
Volume28
Issue number25
DOIs
StatePublished - Jun 7 2010

Fingerprint

Human metapneumovirus
Metapneumovirus
G-proteins
GTP-Binding Proteins
Sigmodon
major surface glycoproteins
Sigmodontinae
Respirovirus
animals
Membrane Glycoproteins
affinity chromatography
Neutralizing Antibodies
Affinity Chromatography
neutralizing antibodies
glycoproteins
Immunization
Glycoproteins
immunization
immune response
Viruses

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Ryder, A., Tollefson, S. J., Podsiad, A. B., Johnson, J. E., & Williams, J. V. (2010). Soluble recombinant human metapneumovirus G protein is immunogenic but not protective. Vaccine, 28(25), 4145-4152. https://doi.org/10.1016/j.vaccine.2010.04.007

Soluble recombinant human metapneumovirus G protein is immunogenic but not protective. / Ryder, Alex; Tollefson, Sharon J.; Podsiad, Amy B.; Johnson, Joyce E.; Williams, John V.

In: Vaccine, Vol. 28, No. 25, 07.06.2010, p. 4145-4152.

Research output: Contribution to journalArticle

Ryder, A, Tollefson, SJ, Podsiad, AB, Johnson, JE & Williams, JV 2010, 'Soluble recombinant human metapneumovirus G protein is immunogenic but not protective', Vaccine, vol. 28, no. 25, pp. 4145-4152. https://doi.org/10.1016/j.vaccine.2010.04.007
Ryder, Alex ; Tollefson, Sharon J. ; Podsiad, Amy B. ; Johnson, Joyce E. ; Williams, John V. / Soluble recombinant human metapneumovirus G protein is immunogenic but not protective. In: Vaccine. 2010 ; Vol. 28, No. 25. pp. 4145-4152.
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