Species-dependent variations in erythrocyte membrane skeletal proteins

C. F. Whitfield, L. M. Mylin, Steven Goodman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Two mammalian species (porcine and murine) have erythrocytes that are being widely used to study membrane protein synthesis and red cell aging. Erythrocytes of these species however, are significantly smaller than those of the human. Before results obtained from study of these red cells can be applied to human cells, the membrane skeleton of these species must be investigated to determine if the skeletal elements are equivalent. Both pig and mouse bands 4.1b were of lower molecular weight than human 4.1b, and the a/b ratio was lower. In each species, 4.1a and b were sequence-related phosphoproteins, and yielded substantially different one-dimensional peptide maps. Band 3 of pig and mouse erythrocytes had a higher molecular weight than human band 3 and also had differing one-dimensional peptide maps after limited proteolytic cleavage with three different enzymes. In each species, free band 3 and band 3 bound to the membrane skeleton had identical peptide maps. Other major membrane skeletal components (spectrin, actin, and bands 2.1 and 4.2) seem to be very similar in molecular weight in various species. These results demonstrate that the molecular weights and relative proportions of the membrane skeletal elements are species dependent.

Original languageEnglish (US)
Pages (from-to)500-506
Number of pages7
JournalBlood
Volume61
Issue number3
StatePublished - Jan 1 1983
Externally publishedYes

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Erythrocyte Membrane
Membrane Proteins
Molecular Weight
Molecular weight
Membranes
Swine
Erythrocytes
Cells
Skeleton
Peptides
Proteins
Spectrin
Cell Aging
Phosphoproteins
Actins
Aging of materials
Cell Membrane
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Species-dependent variations in erythrocyte membrane skeletal proteins. / Whitfield, C. F.; Mylin, L. M.; Goodman, Steven.

In: Blood, Vol. 61, No. 3, 01.01.1983, p. 500-506.

Research output: Contribution to journalArticle

Whitfield, CF, Mylin, LM & Goodman, S 1983, 'Species-dependent variations in erythrocyte membrane skeletal proteins', Blood, vol. 61, no. 3, pp. 500-506.
Whitfield, C. F. ; Mylin, L. M. ; Goodman, Steven. / Species-dependent variations in erythrocyte membrane skeletal proteins. In: Blood. 1983 ; Vol. 61, No. 3. pp. 500-506.
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