Specific cleavage of human type III collagen by human polymorphonuclear leukocyte elastase

C. L. Mainardi, D. L. Hasty, J. M. Seyer, Andrew Kang

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Purified polymorphonuclear leukocyte elastase degraded native human liver type III collagen at 27°C by making a cleavage through the triple helix. The enzyme had no effect on human type I collagen. The reaction was inhibited by phenylmethanesulfonyl fluoride (PhCH2SO2F) but not by EDTA. The collagen reaction products were identical with those generated by rheumatoid synovial collagenase when analyzed by polyacrylamide gel electrophoresis and gel filtration. NH2-terminal sequence analysis indicated that the enzyme cleaved at an isoleucyl-threonyl bond located 4 residues on the carboxyl side of the established cleavage site for animal collagenases. Therefore, it is likely that in pathologic states, type III collagen can be selectively depleted from the matrix by this enzyme.

Original languageEnglish (US)
Pages (from-to)12006-12010
Number of pages5
JournalJournal of Biological Chemistry
Volume255
Issue number24
StatePublished - Dec 1 1980

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Leukocyte Elastase
Collagen Type III
Collagenases
Enzymes
Phenylmethylsulfonyl Fluoride
Collagen Type I
Electrophoresis
Reaction products
Population Groups
Edetic Acid
Liver
Gel Chromatography
Sequence Analysis
Polyacrylamide Gel Electrophoresis
Animals
Collagen
Gels

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Specific cleavage of human type III collagen by human polymorphonuclear leukocyte elastase. / Mainardi, C. L.; Hasty, D. L.; Seyer, J. M.; Kang, Andrew.

In: Journal of Biological Chemistry, Vol. 255, No. 24, 01.12.1980, p. 12006-12010.

Research output: Contribution to journalArticle

Mainardi, C. L. ; Hasty, D. L. ; Seyer, J. M. ; Kang, Andrew. / Specific cleavage of human type III collagen by human polymorphonuclear leukocyte elastase. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 24. pp. 12006-12010.
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