Specific detection of Plasmodium falciparum malaria by a molecularly cloned DNA probe

Ramareddy Guntaka, Arepalli S. Rao, Theodore J. Green, William E. Collins

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A highly repeated DNA sequence from Plasmodium falciparum was cloned and used as a probe in molecular hybridization to detect malaria. Our results indicate that the probe is specific to P. falciparum but not to other species of Plasmodium and is extremely sensitive. As little as a 20 pg parasite DNA, which is equivalent to about 1000 parasites can be detected. The cloned DNA can be used as a diagnostic tool to follow the course of infection of falciparum malaria.

Original languageEnglish (US)
Pages (from-to)363-368
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number1
DOIs
StatePublished - Jul 16 1986
Externally publishedYes

Fingerprint

Falciparum Malaria
DNA Probes
Plasmodium falciparum
Parasites
Molecular Probes
Plasmodium
DNA sequences
DNA
Malaria
Infection

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Specific detection of Plasmodium falciparum malaria by a molecularly cloned DNA probe. / Guntaka, Ramareddy; Rao, Arepalli S.; Green, Theodore J.; Collins, William E.

In: Biochemical and Biophysical Research Communications, Vol. 138, No. 1, 16.07.1986, p. 363-368.

Research output: Contribution to journalArticle

Guntaka, Ramareddy ; Rao, Arepalli S. ; Green, Theodore J. ; Collins, William E. / Specific detection of Plasmodium falciparum malaria by a molecularly cloned DNA probe. In: Biochemical and Biophysical Research Communications. 1986 ; Vol. 138, No. 1. pp. 363-368.
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