Spectrophotometric Assay for Hypochlorite/Hypochlorous Acid Using Tris(2-carboxyethyl)phosphine

Joan Han, Teh Ching Chu, Grace Han, John Browne, Irene Brown, Peter Han

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A simple spectrophotometric method for quantitative determination of hypochlorite (OCl-) or hypochlorous acid (HOCl) is described. The OCl- or HOCl sample is first incubated with an excess amount of tris(2-carboxyethyl)phosphine (TCEP). The concentration of the residual TCEP is then measured as the amount of 2-nitro-5-thiobenzoate produced after reaction with 5,5′-dithiobis(2-nitrobenzoic acid). The concentration of OCl- or HOCl is equivalent to the amount of decrease in the concentration of TCEP because one molecule of TCEP is rapidly and irreversibly oxidized to TCEP oxide by one molecule of OCl- or HOCl. This method is more sensitive and convenient than the standard procedure of NaOCl assay which involves reaction with potassium iodide followed by titration of the liberated triiodide with thiosulfate.

Original languageEnglish (US)
Pages (from-to)218-224
Number of pages7
JournalMicrochemical Journal
Volume58
Issue number2
DOIs
StatePublished - Jan 1 1998

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Hypochlorous Acid
Assays
Nitrobenzoates
Potassium Iodide
Thiosulfates
Molecules
Titration
Oxides
tris(2-carboxyethyl)phosphine

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy

Cite this

Spectrophotometric Assay for Hypochlorite/Hypochlorous Acid Using Tris(2-carboxyethyl)phosphine. / Han, Joan; Chu, Teh Ching; Han, Grace; Browne, John; Brown, Irene; Han, Peter.

In: Microchemical Journal, Vol. 58, No. 2, 01.01.1998, p. 218-224.

Research output: Contribution to journalArticle

Han, Joan ; Chu, Teh Ching ; Han, Grace ; Browne, John ; Brown, Irene ; Han, Peter. / Spectrophotometric Assay for Hypochlorite/Hypochlorous Acid Using Tris(2-carboxyethyl)phosphine. In: Microchemical Journal. 1998 ; Vol. 58, No. 2. pp. 218-224.
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