Spermine, a molecular switch regulating EGFR, integrin β3, Src, and FAK scaffolding

Ramesh M. Ray, Chunying Li, Sujoy Bhattacharya, Anjaparavanda P. Naren, Leonard R. Johnson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Intracellular polyamine levels are highly regulated by the activity of ornithine decarboxylase (ODC), which catalyzes the first rate-limiting reaction in polyamine biosynthesis, producing putrescine, which is subsequently converted to spermidine and spermine. We have shown that polyamines regulate proliferation, migration, and apoptosis in intestinal epithelial cells. Polyamines regulate key signaling events at the level of the EGFR and Src. However, the precise mechanism of action of polyamines is unknown. In the present study, we demonstrate that ODC localizes in lamellipodia and in adhesion plaques during cell spreading. Spermine regulates EGF-induced migration by modulating the interaction of the EGFR with Src. The EGFR interacted with integrin β3, Src, and focal adhesion kinase (FAK). Active Src (pY418-Src) localized with FAK during spreading and migration. Spermine prevented EGF-induced binding of the EGFR with integrin β3, Src, and FAK. Activation of Src and FAK was necessary for EGF-induced migration in HEK293 cells. EGFR-mediated Src activation in live HEK293 cells using a FRET based Src reporter showed that polyamine depletion significantly increased Src kinase activity. In vitro binding studies showed that spermine directly binds Src, and preferentially interacts with the SH2 domain of Src. The physical interaction between Src and the EGFR was severely attenuated by spermine. Therefore, spermine acts as a molecular switch in regulating EGFR-Src coupling both physically and functionally. Upon activation of the EGFR, integrin β3, FAK and Src are recruited to EGFR leading to the trans-activation of both the EGFR and Src and to the Src-mediated phosphorylation of FAK. The activation of FAK induced Rho-GTPases and subsequently migration. This is the first study to define mechanistically how polyamines modulate Src function at the molecular level.

Original languageEnglish (US)
Pages (from-to)931-942
Number of pages12
JournalCellular Signalling
Volume24
Issue number4
DOIs
StatePublished - Apr 1 2012

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Spermine
Polyamines
Integrins
Epidermal Growth Factor
Ornithine Decarboxylase
HEK293 Cells
rho GTP-Binding Proteins
Pseudopodia
Focal Adhesions
Putrescine
Spermidine
src Homology Domains
src-Family Kinases
Epithelial Cells
Phosphorylation
Apoptosis

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Spermine, a molecular switch regulating EGFR, integrin β3, Src, and FAK scaffolding. / Ray, Ramesh M.; Li, Chunying; Bhattacharya, Sujoy; Naren, Anjaparavanda P.; Johnson, Leonard R.

In: Cellular Signalling, Vol. 24, No. 4, 01.04.2012, p. 931-942.

Research output: Contribution to journalArticle

Ray, Ramesh M. ; Li, Chunying ; Bhattacharya, Sujoy ; Naren, Anjaparavanda P. ; Johnson, Leonard R. / Spermine, a molecular switch regulating EGFR, integrin β3, Src, and FAK scaffolding. In: Cellular Signalling. 2012 ; Vol. 24, No. 4. pp. 931-942.
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