Sphingosine 1-phosphate as a major bioactive lysophospholipid that is released from platelets and interacts with endothelial cells

Y. Yatomi, T. Ohmori, G. Rile, F. Kazama, H. Okamoto, T. Sano, K. Satoh, S. Kume, Gabor Tigyi, Y. Igarashi, Y. Ozaki

Research output: Contribution to journalArticle

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Abstract

The serum-borne lysophospholipid mediators sphingosine 1-phosphate (Sph-1-P) and lysophosphatidic acid (LPA) have been shown to be released from activated platelets and to act on endothelial cells. In this study, we employed the repeated lipid extraction (under alkaline and acidic conditions), capable of detecting Sph-1-P, LPA, and possibly structurally similar lysophospholipids, whereby a marked formation of [32P]Sph-1-P, but not [32P]LPA, was observed in [32P]orthophosphate-labeled platelets. Platelet Sph-1-P release, possibly mediated by protein kinase C, was greatly enhanced in the presence of albumin, which formed a complex with Sph-1-P. This finding suggests that platelet Sph-1-P may become accessible to depletion by albumin when its transbilayer movement (flipping) across the plasma membrane is enhanced by protein kinase C. Although human umbilical vein endothelial cells expressed receptors for both Sph-1-P and LPA, Sph-1-P acted much more potently than LPA on the cells in terms of intracellular Ca++ mobilization, cytoskeletal reorganization, and migration. The results suggest that Sph-1-P, rather than LPA, is a major bioactive lysophospholipid that is released from platelets and interacts with endothelial cells, under the conditions in which critical platelet-endothelial interactions (including thrombosis, angiogenesis, and atherosclerosis) occur. Furthermore, albumin-bound Sph-1-P may account for at least some of the serum biological activities on endothelial cells, which have been ascribed to the effects of albumin-bound LPA, based on the similarities between LPA and serum effects. (C) 2000 by The American Society of Hematology.

Original languageEnglish (US)
Pages (from-to)3431-3438
Number of pages8
JournalBlood
Volume96
Issue number10
StatePublished - Nov 15 2000
Externally publishedYes

Fingerprint

Lysophospholipids
Endothelial cells
Platelets
Blood Platelets
Endothelial Cells
Albumins
Protein Kinase C
Serum
Lysosphingolipid Receptors
sphingosine 1-phosphate
lysophosphatidic acid
Human Umbilical Vein Endothelial Cells
Cell membranes
Bioactivity
Atherosclerosis
Thrombosis
Phosphates
Cell Membrane
Lipids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Yatomi, Y., Ohmori, T., Rile, G., Kazama, F., Okamoto, H., Sano, T., ... Ozaki, Y. (2000). Sphingosine 1-phosphate as a major bioactive lysophospholipid that is released from platelets and interacts with endothelial cells. Blood, 96(10), 3431-3438.

Sphingosine 1-phosphate as a major bioactive lysophospholipid that is released from platelets and interacts with endothelial cells. / Yatomi, Y.; Ohmori, T.; Rile, G.; Kazama, F.; Okamoto, H.; Sano, T.; Satoh, K.; Kume, S.; Tigyi, Gabor; Igarashi, Y.; Ozaki, Y.

In: Blood, Vol. 96, No. 10, 15.11.2000, p. 3431-3438.

Research output: Contribution to journalArticle

Yatomi, Y, Ohmori, T, Rile, G, Kazama, F, Okamoto, H, Sano, T, Satoh, K, Kume, S, Tigyi, G, Igarashi, Y & Ozaki, Y 2000, 'Sphingosine 1-phosphate as a major bioactive lysophospholipid that is released from platelets and interacts with endothelial cells', Blood, vol. 96, no. 10, pp. 3431-3438.
Yatomi Y, Ohmori T, Rile G, Kazama F, Okamoto H, Sano T et al. Sphingosine 1-phosphate as a major bioactive lysophospholipid that is released from platelets and interacts with endothelial cells. Blood. 2000 Nov 15;96(10):3431-3438.
Yatomi, Y. ; Ohmori, T. ; Rile, G. ; Kazama, F. ; Okamoto, H. ; Sano, T. ; Satoh, K. ; Kume, S. ; Tigyi, Gabor ; Igarashi, Y. ; Ozaki, Y. / Sphingosine 1-phosphate as a major bioactive lysophospholipid that is released from platelets and interacts with endothelial cells. In: Blood. 2000 ; Vol. 96, No. 10. pp. 3431-3438.
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AU - Rile, G.

AU - Kazama, F.

AU - Okamoto, H.

AU - Sano, T.

AU - Satoh, K.

AU - Kume, S.

AU - Tigyi, Gabor

AU - Igarashi, Y.

AU - Ozaki, Y.

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N2 - The serum-borne lysophospholipid mediators sphingosine 1-phosphate (Sph-1-P) and lysophosphatidic acid (LPA) have been shown to be released from activated platelets and to act on endothelial cells. In this study, we employed the repeated lipid extraction (under alkaline and acidic conditions), capable of detecting Sph-1-P, LPA, and possibly structurally similar lysophospholipids, whereby a marked formation of [32P]Sph-1-P, but not [32P]LPA, was observed in [32P]orthophosphate-labeled platelets. Platelet Sph-1-P release, possibly mediated by protein kinase C, was greatly enhanced in the presence of albumin, which formed a complex with Sph-1-P. This finding suggests that platelet Sph-1-P may become accessible to depletion by albumin when its transbilayer movement (flipping) across the plasma membrane is enhanced by protein kinase C. Although human umbilical vein endothelial cells expressed receptors for both Sph-1-P and LPA, Sph-1-P acted much more potently than LPA on the cells in terms of intracellular Ca++ mobilization, cytoskeletal reorganization, and migration. The results suggest that Sph-1-P, rather than LPA, is a major bioactive lysophospholipid that is released from platelets and interacts with endothelial cells, under the conditions in which critical platelet-endothelial interactions (including thrombosis, angiogenesis, and atherosclerosis) occur. Furthermore, albumin-bound Sph-1-P may account for at least some of the serum biological activities on endothelial cells, which have been ascribed to the effects of albumin-bound LPA, based on the similarities between LPA and serum effects. (C) 2000 by The American Society of Hematology.

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