Spontaneous fracture (sfx)

A mouse genetic model of defective peripubertal bone formation

W. G. Beamer, C. J. Rosen, R. T. Bronson, Weikuan Gu, L. R. Donahue, D. J. Baylink, C. C. Richardson, G. C. Crawford, J. E. Barker

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A new mouse model of stage-specific bone growth failure and fracture has been recovered as an autosomal recessive mutation, designated spontaneous fracture (sfx). The sfx/sfx mice are phenotypically normal until shortly after weaning, when reduced mobility and impaired somatic growth are first noted. By 6 weeks of age, body, spleen, and thymus weights, as well as hematocrits and serum calcium, inorganic phosphate, total alkaline phosphatase, insulin-like growth factor-I, and osteocalcin levels are decreased. The sfx/sfx mice also show reduced femoral cortical density and diaphyseal circumference, as well as a paucity of mature osteoblasts on bone surfaces. Histological analyses of the femur and tibia in the mutants show subtle reduction of chondrocyte numbers in epiphyseal-plate columns, reduction of matrix, and near absence of osteoid below the differentiated chondrocytes. Trabeculae in proximal tibiae, iliacs, and vertebral bodies are sparse and thin. Cortical bone thickness of mutants is markedly thinned in all sites examined. By 7-8 weeks, radiographic films routinely show spontaneous impact fractures of the distal femur accompanied by callus formation, whereas complete fractures are less commonly observed. Volumetric bone mineral density (BMD) of mutant femurs is similar to +/? littermates in the center of the femoral diaphysis, but BMD declines as either end of the femoral diaphysis is approached. We have mapped the gene responsible for this phenotype to central Chromosome 14. Reduced bone mass, impaired bone formation, abnormalities of bone architecture, and a disposition to spontaneous fracture identify sfx/sfx mice as a useful model for understanding the mechanisms responsible for peripubertal bone formation. (C) 2000 by Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)619-626
Number of pages8
JournalBone
Volume27
Issue number5
DOIs
StatePublished - Nov 13 2000

Fingerprint

Spontaneous Fractures
Genetic Models
Osteogenesis
Thigh
Femur
Diaphyses
Chondrocytes
Tibia
Bone and Bones
Bone Density
Chromosomes, Human, Pair 14
X-Ray Film
Growth Plate
Bone Development
Osteocalcin
Bone Fractures
Bony Callus
Weaning
Osteoblasts
Insulin-Like Growth Factor I

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Beamer, W. G., Rosen, C. J., Bronson, R. T., Gu, W., Donahue, L. R., Baylink, D. J., ... Barker, J. E. (2000). Spontaneous fracture (sfx): A mouse genetic model of defective peripubertal bone formation. Bone, 27(5), 619-626. https://doi.org/10.1016/S8756-3282(00)00369-0

Spontaneous fracture (sfx) : A mouse genetic model of defective peripubertal bone formation. / Beamer, W. G.; Rosen, C. J.; Bronson, R. T.; Gu, Weikuan; Donahue, L. R.; Baylink, D. J.; Richardson, C. C.; Crawford, G. C.; Barker, J. E.

In: Bone, Vol. 27, No. 5, 13.11.2000, p. 619-626.

Research output: Contribution to journalArticle

Beamer, WG, Rosen, CJ, Bronson, RT, Gu, W, Donahue, LR, Baylink, DJ, Richardson, CC, Crawford, GC & Barker, JE 2000, 'Spontaneous fracture (sfx): A mouse genetic model of defective peripubertal bone formation', Bone, vol. 27, no. 5, pp. 619-626. https://doi.org/10.1016/S8756-3282(00)00369-0
Beamer, W. G. ; Rosen, C. J. ; Bronson, R. T. ; Gu, Weikuan ; Donahue, L. R. ; Baylink, D. J. ; Richardson, C. C. ; Crawford, G. C. ; Barker, J. E. / Spontaneous fracture (sfx) : A mouse genetic model of defective peripubertal bone formation. In: Bone. 2000 ; Vol. 27, No. 5. pp. 619-626.
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AU - Donahue, L. R.

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