SRF and TEF-1 control of chicken skeletal α-actin gene during slow- muscle hypertrophy

James Carson, Robert J. Schwartz, Frank W. Booth

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The purpose of this study was to delineate the α-actin regulatory elements and transcription factors that are responsible for conferring stretch-overload responsiveness during hypertrophy of the anterior latissimus dorsi (ALD) muscle of young chickens by weighting one wing. Minimal promoter constructs were evaluated by direct injection into the ALD, which demonstrated that both serum response element 1 (SRE1) and the transcriptional enhancer factor 1 (TEF-1) elements were sufficient for increased expression during stretch overload. A mutated SRE1 prevented expression in both basal and stretched ALD muscles, whereas a mutated TEF-1 element reduced actin promoter function in both control and stretched muscles. The serum response factor (SRF)-SRE1 binding complex demonstrated faster migration in mobility shift assays from day 3- and day 6-stretched ALD nuclear extracts relative to their control. TEF-1 binding was qualitatively increased in stretched extracts at day 3 but not day 6 of stretch overload. Skeletal α-actin mRNA accumulated from day 3 to day 6 of stretch overload. These data demonstrate that SRE1 is necessary and sufficient for stretch- overload responsiveness from the skeletal α-actin promoter and that the SRF- SRE1 binding complex migrates faster in stretched nuclear extracts of hypertrophied relative to control extracts from intact ALD muscles of chickens.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume270
Issue number6 39-6
StatePublished - Jun 1 1996

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Serum Response Element
Serum Response Factor
Superficial Back Muscles
Hypertrophy
Actins
Chickens
Muscles
Genes
Electrophoretic Mobility Shift Assay
Transcription Factors
Messenger RNA
Injections

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

SRF and TEF-1 control of chicken skeletal α-actin gene during slow- muscle hypertrophy. / Carson, James; Schwartz, Robert J.; Booth, Frank W.

In: American Journal of Physiology - Cell Physiology, Vol. 270, No. 6 39-6, 01.06.1996.

Research output: Contribution to journalArticle

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