SRF protein is upregulated during stretch-induced hypertrophy of rooster ALD muscle

Martin Flück, James Carson, Robert J. Schwartz, Frank W. Booth

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Serum response element 1 has previously been reported to be necessary and sufficient for activation of the skeletal α-actin promoter during hypertrophy of the anterior latissimus dorsi (ALD) muscle of roosters [J. A. Carson, R. J. Schwartz, and F. W. Booth. Am. J. Physiol. 270 (Cell Physiol. 39): C1624-C1633, 1996]. Serum response factor (SRF) protein is the transcription factor that binds as a homodimer to serum response element 1 and activates the skeletal α-actin promoter. An increased expression of exogenous SRF protein in replicating C2C12 myoblasts induced a three- to fourfold activation of the skeletal α-actin promoter (L. Wei, W. Zhou, J. D. Croissant, F.-E. Johansen, R. Prywes, A. Balasubramamyan, and R. J. Schwartz. J. Biol. Chem. 273: 30287-30294, 1998). Thus we hypothesized that SRF protein concentration would be increased during hypertrophy of skeletal muscle. In the present study, 10% of the rooster's body weight was attached to the left wing to induce enlargement of the ALD muscle compared with the contralateral muscle. With Western analysis, a significant increase in SRF protein per gram of wet weight of the ALD muscle was noted at 7 and 13 days of hypertrophy. Furthermore, the increase in SRF protein occurred in both crude nuclear protein and cytoplasmic fractions in 7-day stretched ALD muscles. This is the first report showing increased protein concentration for a transcription factor whose regulatory element in the skeletal α-actin promoter has previously been shown to be required for the transduction of a hypertrophy signal in overloaded skeletal muscle of an animal.

Original languageEnglish (US)
Pages (from-to)1793-1799
Number of pages7
JournalJournal of applied physiology
Volume86
Issue number6
DOIs
StatePublished - Jan 1 1999

Fingerprint

Serum Response Factor
Superficial Back Muscles
Hypertrophy
Muscles
Actins
Serum Response Element
Proteins
Skeletal Muscle
Transcription Factors
Myoblasts
Nuclear Proteins
Body Weight
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

SRF protein is upregulated during stretch-induced hypertrophy of rooster ALD muscle. / Flück, Martin; Carson, James; Schwartz, Robert J.; Booth, Frank W.

In: Journal of applied physiology, Vol. 86, No. 6, 01.01.1999, p. 1793-1799.

Research output: Contribution to journalArticle

Flück, Martin ; Carson, James ; Schwartz, Robert J. ; Booth, Frank W. / SRF protein is upregulated during stretch-induced hypertrophy of rooster ALD muscle. In: Journal of applied physiology. 1999 ; Vol. 86, No. 6. pp. 1793-1799.
@article{0400f7ab7c404bb7b7045b5a44e1ac0b,
title = "SRF protein is upregulated during stretch-induced hypertrophy of rooster ALD muscle",
abstract = "Serum response element 1 has previously been reported to be necessary and sufficient for activation of the skeletal α-actin promoter during hypertrophy of the anterior latissimus dorsi (ALD) muscle of roosters [J. A. Carson, R. J. Schwartz, and F. W. Booth. Am. J. Physiol. 270 (Cell Physiol. 39): C1624-C1633, 1996]. Serum response factor (SRF) protein is the transcription factor that binds as a homodimer to serum response element 1 and activates the skeletal α-actin promoter. An increased expression of exogenous SRF protein in replicating C2C12 myoblasts induced a three- to fourfold activation of the skeletal α-actin promoter (L. Wei, W. Zhou, J. D. Croissant, F.-E. Johansen, R. Prywes, A. Balasubramamyan, and R. J. Schwartz. J. Biol. Chem. 273: 30287-30294, 1998). Thus we hypothesized that SRF protein concentration would be increased during hypertrophy of skeletal muscle. In the present study, 10{\%} of the rooster's body weight was attached to the left wing to induce enlargement of the ALD muscle compared with the contralateral muscle. With Western analysis, a significant increase in SRF protein per gram of wet weight of the ALD muscle was noted at 7 and 13 days of hypertrophy. Furthermore, the increase in SRF protein occurred in both crude nuclear protein and cytoplasmic fractions in 7-day stretched ALD muscles. This is the first report showing increased protein concentration for a transcription factor whose regulatory element in the skeletal α-actin promoter has previously been shown to be required for the transduction of a hypertrophy signal in overloaded skeletal muscle of an animal.",
author = "Martin Fl{\"u}ck and James Carson and Schwartz, {Robert J.} and Booth, {Frank W.}",
year = "1999",
month = "1",
day = "1",
doi = "10.1152/jappl.1999.86.6.1793",
language = "English (US)",
volume = "86",
pages = "1793--1799",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "6",

}

TY - JOUR

T1 - SRF protein is upregulated during stretch-induced hypertrophy of rooster ALD muscle

AU - Flück, Martin

AU - Carson, James

AU - Schwartz, Robert J.

AU - Booth, Frank W.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Serum response element 1 has previously been reported to be necessary and sufficient for activation of the skeletal α-actin promoter during hypertrophy of the anterior latissimus dorsi (ALD) muscle of roosters [J. A. Carson, R. J. Schwartz, and F. W. Booth. Am. J. Physiol. 270 (Cell Physiol. 39): C1624-C1633, 1996]. Serum response factor (SRF) protein is the transcription factor that binds as a homodimer to serum response element 1 and activates the skeletal α-actin promoter. An increased expression of exogenous SRF protein in replicating C2C12 myoblasts induced a three- to fourfold activation of the skeletal α-actin promoter (L. Wei, W. Zhou, J. D. Croissant, F.-E. Johansen, R. Prywes, A. Balasubramamyan, and R. J. Schwartz. J. Biol. Chem. 273: 30287-30294, 1998). Thus we hypothesized that SRF protein concentration would be increased during hypertrophy of skeletal muscle. In the present study, 10% of the rooster's body weight was attached to the left wing to induce enlargement of the ALD muscle compared with the contralateral muscle. With Western analysis, a significant increase in SRF protein per gram of wet weight of the ALD muscle was noted at 7 and 13 days of hypertrophy. Furthermore, the increase in SRF protein occurred in both crude nuclear protein and cytoplasmic fractions in 7-day stretched ALD muscles. This is the first report showing increased protein concentration for a transcription factor whose regulatory element in the skeletal α-actin promoter has previously been shown to be required for the transduction of a hypertrophy signal in overloaded skeletal muscle of an animal.

AB - Serum response element 1 has previously been reported to be necessary and sufficient for activation of the skeletal α-actin promoter during hypertrophy of the anterior latissimus dorsi (ALD) muscle of roosters [J. A. Carson, R. J. Schwartz, and F. W. Booth. Am. J. Physiol. 270 (Cell Physiol. 39): C1624-C1633, 1996]. Serum response factor (SRF) protein is the transcription factor that binds as a homodimer to serum response element 1 and activates the skeletal α-actin promoter. An increased expression of exogenous SRF protein in replicating C2C12 myoblasts induced a three- to fourfold activation of the skeletal α-actin promoter (L. Wei, W. Zhou, J. D. Croissant, F.-E. Johansen, R. Prywes, A. Balasubramamyan, and R. J. Schwartz. J. Biol. Chem. 273: 30287-30294, 1998). Thus we hypothesized that SRF protein concentration would be increased during hypertrophy of skeletal muscle. In the present study, 10% of the rooster's body weight was attached to the left wing to induce enlargement of the ALD muscle compared with the contralateral muscle. With Western analysis, a significant increase in SRF protein per gram of wet weight of the ALD muscle was noted at 7 and 13 days of hypertrophy. Furthermore, the increase in SRF protein occurred in both crude nuclear protein and cytoplasmic fractions in 7-day stretched ALD muscles. This is the first report showing increased protein concentration for a transcription factor whose regulatory element in the skeletal α-actin promoter has previously been shown to be required for the transduction of a hypertrophy signal in overloaded skeletal muscle of an animal.

UR - http://www.scopus.com/inward/record.url?scp=0344224195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344224195&partnerID=8YFLogxK

U2 - 10.1152/jappl.1999.86.6.1793

DO - 10.1152/jappl.1999.86.6.1793

M3 - Article

VL - 86

SP - 1793

EP - 1799

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 6

ER -