Standardization of acid hydrolysis procedure for urinary 3-methylhistidine determination by high-performance liquid chromatography

David A. Kuhl, J. Travis Methvin, Roland Dickerson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The N-acetylated form of N-methylhistidine (3-methylhistidine, 3-meH), a non-invasive marker of proteolysis, accounts for 80-90% of total 3-meH excretion (acetylated + non-acetylated 3-meH) in the rat. To determine total 3-meH excretion, samples require acid hydrolysis prior to determination by high-performance liquid chromatography. This study evaluated the stability of 3-meH at various times and temperatures of hydrolysis and determined the optimal conditions for hydrolysis of samples. Increasing temperature (120°C) results in significant degradation of 3-meH with no appreciable change in concentration being noted at 80°C. Hydrolysis at 100°C for 1.5 to 4 h or 80°C for 8 to 12 h is recommended for determining total 3-meH concentrations in rat urine.

Original languageEnglish (US)
Pages (from-to)390-394
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Volume681
Issue number2
DOIs
StatePublished - Jun 7 1996

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High performance liquid chromatography
Standardization
Hydrolysis
Acids
Rats
Proteolysis
3-methylhistidine
Degradation
Temperature

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Standardization of acid hydrolysis procedure for urinary 3-methylhistidine determination by high-performance liquid chromatography. / Kuhl, David A.; Methvin, J. Travis; Dickerson, Roland.

In: Journal of Chromatography B: Biomedical Applications, Vol. 681, No. 2, 07.06.1996, p. 390-394.

Research output: Contribution to journalArticle

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