Stem cell repair of physeal cartilage

Jae I. Ahn, S. Terry Canale, Stephanie D. Butler, Karen Hasty

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To evaluate the ability of cultured mesenchymal stem cells (MSC) to repair physeal defects, MSC-matrix constructs with 5% gelatin (group A), 10% gelatin/Gelfoam (Pharmacia, Peapack, NJ) (group B), and MSC grown in the presence of TGF-β3 with Gelfoam (group C) were implanted in proximal tibial physeal defects created in 20 immature rabbits. Control groups (untreated partial defect and partial defect treated with Gelfoam) showed bony bar formation with varus deformities of 30° and 28°, respectively. Group A had an average 23° varus deformity with bony bridge formation, and group B had mild varus angulation (average 14°) of the proximal tibia. In group C, there was no significant varus deformity (average 9°), and histologic examination showed that some of the columnation areas interspersed with chondrocytes were irregularly arranged in the matrix. These findings suggest that repair of physeal defects can be enhanced by the implantation of MSC cultured with TGF-β3.

Original languageEnglish (US)
Pages (from-to)1215-1221
Number of pages7
JournalJournal of Orthopaedic Research
Volume22
Issue number6
DOIs
StatePublished - Nov 1 2004

Fingerprint

Absorbable Gelatin Sponge
Mesenchymal Stromal Cells
Cartilage
Stem Cells
Gelatin
Chondrocytes
Tibia
Rabbits
Control Groups

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Cite this

Stem cell repair of physeal cartilage. / Ahn, Jae I.; Canale, S. Terry; Butler, Stephanie D.; Hasty, Karen.

In: Journal of Orthopaedic Research, Vol. 22, No. 6, 01.11.2004, p. 1215-1221.

Research output: Contribution to journalArticle

Ahn, Jae I. ; Canale, S. Terry ; Butler, Stephanie D. ; Hasty, Karen. / Stem cell repair of physeal cartilage. In: Journal of Orthopaedic Research. 2004 ; Vol. 22, No. 6. pp. 1215-1221.
@article{a46b3492b8694fbda87ff3213c94a52f,
title = "Stem cell repair of physeal cartilage",
abstract = "To evaluate the ability of cultured mesenchymal stem cells (MSC) to repair physeal defects, MSC-matrix constructs with 5{\%} gelatin (group A), 10{\%} gelatin/Gelfoam (Pharmacia, Peapack, NJ) (group B), and MSC grown in the presence of TGF-β3 with Gelfoam (group C) were implanted in proximal tibial physeal defects created in 20 immature rabbits. Control groups (untreated partial defect and partial defect treated with Gelfoam) showed bony bar formation with varus deformities of 30° and 28°, respectively. Group A had an average 23° varus deformity with bony bridge formation, and group B had mild varus angulation (average 14°) of the proximal tibia. In group C, there was no significant varus deformity (average 9°), and histologic examination showed that some of the columnation areas interspersed with chondrocytes were irregularly arranged in the matrix. These findings suggest that repair of physeal defects can be enhanced by the implantation of MSC cultured with TGF-β3.",
author = "Ahn, {Jae I.} and Canale, {S. Terry} and Butler, {Stephanie D.} and Karen Hasty",
year = "2004",
month = "11",
day = "1",
doi = "10.1016/j.orthres.2004.03.012",
language = "English (US)",
volume = "22",
pages = "1215--1221",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Stem cell repair of physeal cartilage

AU - Ahn, Jae I.

AU - Canale, S. Terry

AU - Butler, Stephanie D.

AU - Hasty, Karen

PY - 2004/11/1

Y1 - 2004/11/1

N2 - To evaluate the ability of cultured mesenchymal stem cells (MSC) to repair physeal defects, MSC-matrix constructs with 5% gelatin (group A), 10% gelatin/Gelfoam (Pharmacia, Peapack, NJ) (group B), and MSC grown in the presence of TGF-β3 with Gelfoam (group C) were implanted in proximal tibial physeal defects created in 20 immature rabbits. Control groups (untreated partial defect and partial defect treated with Gelfoam) showed bony bar formation with varus deformities of 30° and 28°, respectively. Group A had an average 23° varus deformity with bony bridge formation, and group B had mild varus angulation (average 14°) of the proximal tibia. In group C, there was no significant varus deformity (average 9°), and histologic examination showed that some of the columnation areas interspersed with chondrocytes were irregularly arranged in the matrix. These findings suggest that repair of physeal defects can be enhanced by the implantation of MSC cultured with TGF-β3.

AB - To evaluate the ability of cultured mesenchymal stem cells (MSC) to repair physeal defects, MSC-matrix constructs with 5% gelatin (group A), 10% gelatin/Gelfoam (Pharmacia, Peapack, NJ) (group B), and MSC grown in the presence of TGF-β3 with Gelfoam (group C) were implanted in proximal tibial physeal defects created in 20 immature rabbits. Control groups (untreated partial defect and partial defect treated with Gelfoam) showed bony bar formation with varus deformities of 30° and 28°, respectively. Group A had an average 23° varus deformity with bony bridge formation, and group B had mild varus angulation (average 14°) of the proximal tibia. In group C, there was no significant varus deformity (average 9°), and histologic examination showed that some of the columnation areas interspersed with chondrocytes were irregularly arranged in the matrix. These findings suggest that repair of physeal defects can be enhanced by the implantation of MSC cultured with TGF-β3.

UR - http://www.scopus.com/inward/record.url?scp=4844226855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4844226855&partnerID=8YFLogxK

U2 - 10.1016/j.orthres.2004.03.012

DO - 10.1016/j.orthres.2004.03.012

M3 - Article

VL - 22

SP - 1215

EP - 1221

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 6

ER -