Sterilization by cooling in isochoric conditions

The case of Escherichia coli

Samuel Salinas-Almaguer, Abril Angulo-Sherman, Francisco Sierra Valdez, Hilda Mercado-Uribe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

High hydrostatic pressure (HHP) affects the structure, metabolism and survival of microorganisms including bacteria. For this reason HHP is a promising treatment in the food industry. The aim of this work is to evaluate the effect of high pressure, under isochoric cooling conditions, on Escherichia coli, where such high pressure develops due to the fact water cannot expand. We combine survival curves obtained by spectrophotometry and images of atomic force microscopy in this study. Our results show that cooling at-20 and-30°C leads to a partial destruction of a Escherichia coli population. However, cooling at-15°C causes a total extermination of bacteria. This intriguing result is explained by the phase diagram of water. In the first case, the simultaneous formation of ice III and ice Ih crystals provides a safe environment for bacteria. In the second case (-15°C) Escherichia coli remains in a metastable and amorphous free-of-crystals liquid subjected to high pressure. Our work is the first experimental study carried out to inactivate Escherichia coli under isochoric cooling conditions. Unlike HHP, which is based on the application of an external load to augment the pressure, this technique only requires cooling. The method could be used for annihilation of other Escherichia coli strains and perhaps other micro-organisms.

Original languageEnglish (US)
Article numbere0140882
JournalPLoS ONE
Volume10
Issue number10
DOIs
StatePublished - Oct 19 2015

Fingerprint

Escherichia coli
cooling
Hydrostatic Pressure
Cooling
Hydrostatic pressure
Pressure
Bacteria
Ice
Liquid Crystals
bacteria
Water
Atomic Force Microscopy
Food Industry
Spectrophotometry
ice
Metabolism
Microorganisms
microorganisms
Phase diagrams
Atomic force microscopy

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Salinas-Almaguer, S., Angulo-Sherman, A., Sierra Valdez, F., & Mercado-Uribe, H. (2015). Sterilization by cooling in isochoric conditions: The case of Escherichia coli. PLoS ONE, 10(10), [e0140882]. https://doi.org/10.1371/journal.pone.0140882

Sterilization by cooling in isochoric conditions : The case of Escherichia coli. / Salinas-Almaguer, Samuel; Angulo-Sherman, Abril; Sierra Valdez, Francisco; Mercado-Uribe, Hilda.

In: PLoS ONE, Vol. 10, No. 10, e0140882, 19.10.2015.

Research output: Contribution to journalArticle

Salinas-Almaguer, S, Angulo-Sherman, A, Sierra Valdez, F & Mercado-Uribe, H 2015, 'Sterilization by cooling in isochoric conditions: The case of Escherichia coli', PLoS ONE, vol. 10, no. 10, e0140882. https://doi.org/10.1371/journal.pone.0140882
Salinas-Almaguer S, Angulo-Sherman A, Sierra Valdez F, Mercado-Uribe H. Sterilization by cooling in isochoric conditions: The case of Escherichia coli. PLoS ONE. 2015 Oct 19;10(10). e0140882. https://doi.org/10.1371/journal.pone.0140882
Salinas-Almaguer, Samuel ; Angulo-Sherman, Abril ; Sierra Valdez, Francisco ; Mercado-Uribe, Hilda. / Sterilization by cooling in isochoric conditions : The case of Escherichia coli. In: PLoS ONE. 2015 ; Vol. 10, No. 10.
@article{f37fdbaf435d4fefb4433a50412162e9,
title = "Sterilization by cooling in isochoric conditions: The case of Escherichia coli",
abstract = "High hydrostatic pressure (HHP) affects the structure, metabolism and survival of microorganisms including bacteria. For this reason HHP is a promising treatment in the food industry. The aim of this work is to evaluate the effect of high pressure, under isochoric cooling conditions, on Escherichia coli, where such high pressure develops due to the fact water cannot expand. We combine survival curves obtained by spectrophotometry and images of atomic force microscopy in this study. Our results show that cooling at-20 and-30°C leads to a partial destruction of a Escherichia coli population. However, cooling at-15°C causes a total extermination of bacteria. This intriguing result is explained by the phase diagram of water. In the first case, the simultaneous formation of ice III and ice Ih crystals provides a safe environment for bacteria. In the second case (-15°C) Escherichia coli remains in a metastable and amorphous free-of-crystals liquid subjected to high pressure. Our work is the first experimental study carried out to inactivate Escherichia coli under isochoric cooling conditions. Unlike HHP, which is based on the application of an external load to augment the pressure, this technique only requires cooling. The method could be used for annihilation of other Escherichia coli strains and perhaps other micro-organisms.",
author = "Samuel Salinas-Almaguer and Abril Angulo-Sherman and {Sierra Valdez}, Francisco and Hilda Mercado-Uribe",
year = "2015",
month = "10",
day = "19",
doi = "10.1371/journal.pone.0140882",
language = "English (US)",
volume = "10",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

TY - JOUR

T1 - Sterilization by cooling in isochoric conditions

T2 - The case of Escherichia coli

AU - Salinas-Almaguer, Samuel

AU - Angulo-Sherman, Abril

AU - Sierra Valdez, Francisco

AU - Mercado-Uribe, Hilda

PY - 2015/10/19

Y1 - 2015/10/19

N2 - High hydrostatic pressure (HHP) affects the structure, metabolism and survival of microorganisms including bacteria. For this reason HHP is a promising treatment in the food industry. The aim of this work is to evaluate the effect of high pressure, under isochoric cooling conditions, on Escherichia coli, where such high pressure develops due to the fact water cannot expand. We combine survival curves obtained by spectrophotometry and images of atomic force microscopy in this study. Our results show that cooling at-20 and-30°C leads to a partial destruction of a Escherichia coli population. However, cooling at-15°C causes a total extermination of bacteria. This intriguing result is explained by the phase diagram of water. In the first case, the simultaneous formation of ice III and ice Ih crystals provides a safe environment for bacteria. In the second case (-15°C) Escherichia coli remains in a metastable and amorphous free-of-crystals liquid subjected to high pressure. Our work is the first experimental study carried out to inactivate Escherichia coli under isochoric cooling conditions. Unlike HHP, which is based on the application of an external load to augment the pressure, this technique only requires cooling. The method could be used for annihilation of other Escherichia coli strains and perhaps other micro-organisms.

AB - High hydrostatic pressure (HHP) affects the structure, metabolism and survival of microorganisms including bacteria. For this reason HHP is a promising treatment in the food industry. The aim of this work is to evaluate the effect of high pressure, under isochoric cooling conditions, on Escherichia coli, where such high pressure develops due to the fact water cannot expand. We combine survival curves obtained by spectrophotometry and images of atomic force microscopy in this study. Our results show that cooling at-20 and-30°C leads to a partial destruction of a Escherichia coli population. However, cooling at-15°C causes a total extermination of bacteria. This intriguing result is explained by the phase diagram of water. In the first case, the simultaneous formation of ice III and ice Ih crystals provides a safe environment for bacteria. In the second case (-15°C) Escherichia coli remains in a metastable and amorphous free-of-crystals liquid subjected to high pressure. Our work is the first experimental study carried out to inactivate Escherichia coli under isochoric cooling conditions. Unlike HHP, which is based on the application of an external load to augment the pressure, this technique only requires cooling. The method could be used for annihilation of other Escherichia coli strains and perhaps other micro-organisms.

UR - http://www.scopus.com/inward/record.url?scp=84949033993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949033993&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0140882

DO - 10.1371/journal.pone.0140882

M3 - Article

VL - 10

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 10

M1 - e0140882

ER -