Sterilization of Mycobacterium tuberculosis infected samples using methanol preserves anti-tuberculosis drugs for subsequent pharmacological testing studies

Amanda Walz, Pradeep B. Lukka, Camron Pearce, Elizabeth Creissen, Miriam Braunstein, Anthony J. Hickey, Bernd Meibohm, Mercedes Gonzalez-Juarrero

Research output: Contribution to journalArticle

Abstract

Pharmacokinetic/pharmacodynamic studies of anti-tuberculosis agents in animal models of tuberculosis are hampered by the frequent necessity to perform sample bioanalysis outside the biosafety level-3 environment. Thus, each specimen has to undergo tedious and time-consuming sample sterilization procedures that may also affect drug stability. Here, we tested treatment of Mycobacterium tuberculosis (Mtb) infected samples with methanol to sterilize samples while preserving drug integrity for further pharmacokinetic/pharmacodynamic evaluations. Tissue samples harvested from Mtb infected mice were homogenized, incubated in methanol, and tested for sterility. Once sterility was confirmed, the samples were used to determine concentrations of the anti-tuberculosis drug spectinamide-1599 in lung homogenates using liquid chromatography coupled with mass spectrometry. The results demonstrate that methanol sterilizes tissue samples harvested from Mtb infected mice without altering the integrity of the drug in the tissue.

Original languageEnglish (US)
Pages (from-to)52-55
Number of pages4
JournalTuberculosis
Volume117
DOIs
StatePublished - Jul 1 2019

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Mycobacterium tuberculosis
Methanol
Tuberculosis
Pharmacology
Infertility
Pharmacokinetics
Pharmaceutical Preparations
Drug Stability
Liquid Chromatography
Mass Spectrometry
Animal Models
Lung

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Sterilization of Mycobacterium tuberculosis infected samples using methanol preserves anti-tuberculosis drugs for subsequent pharmacological testing studies. / Walz, Amanda; Lukka, Pradeep B.; Pearce, Camron; Creissen, Elizabeth; Braunstein, Miriam; Hickey, Anthony J.; Meibohm, Bernd; Gonzalez-Juarrero, Mercedes.

In: Tuberculosis, Vol. 117, 01.07.2019, p. 52-55.

Research output: Contribution to journalArticle

Walz, Amanda ; Lukka, Pradeep B. ; Pearce, Camron ; Creissen, Elizabeth ; Braunstein, Miriam ; Hickey, Anthony J. ; Meibohm, Bernd ; Gonzalez-Juarrero, Mercedes. / Sterilization of Mycobacterium tuberculosis infected samples using methanol preserves anti-tuberculosis drugs for subsequent pharmacological testing studies. In: Tuberculosis. 2019 ; Vol. 117. pp. 52-55.
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