Stimulation of leptin release by growth hormone in rat adipose tissue

J. N. Fain, Suleiman Bahouth

Research output: Contribution to journalArticle

Abstract

In cultured adipose tissue from fed hypothyroid rats the release of leptin was enhanced by dexamethasone between 6 and 24 h of incubation and further elevated by the combination of insulin plus dexamethasone. There was a loss of leptin mRNA at 6 or 24 h that was largely prevented by dexamethasone. While insulin enhanced leptin release and GAPDH mRNA it reduced the ability of dexamethasone to prevent leptin mRNA loss over 24 h. The specific β3 catecholamine agonist Cl 316,243 inhibited dexamethasone induced leptin release and leptin mRNA accumulation. In contrast growth hormone (GH) increased leptin release in the absence of insulin while partially blocking the ability of dexamethasone to prevent loss of leptin mRNA over 24 h. In the presence of insulin or triiodothyronine plus dexamethasone an increase in both leptin release and leptin mRNA was seen with GH. The maximal effect of GH on leptin mRNA was seen with 1 nM GH and at 6 h while stimulation of leptin release was not seen until 24 h. These data indicate a new role for GH as a stimulator of leptin release in rat adipose tissue.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number8
StatePublished - 1998

Fingerprint

Leptin
leptin
somatotropin
Growth Hormone
adipose tissue
Adipose Tissue
Rats
Tissue
dexamethasone
rats
Dexamethasone
Messenger RNA
insulin
Insulin
Triiodothyronine
triiodothyronine
catecholamines
agonists
Catecholamines

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Stimulation of leptin release by growth hormone in rat adipose tissue. / Fain, J. N.; Bahouth, Suleiman.

In: FASEB Journal, Vol. 12, No. 8, 1998.

Research output: Contribution to journalArticle

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