Strain differences in allele and expression levels of CD72 on B-lymphocytes from NOD, AKR, NON and C57BL/6 mice

Jian Wu, Jacob Marler, Nataliya I. Lenchik, Ivan Gerling

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2 Citations (Scopus)

Abstract

The B-lymphocyte marker CD72 has multiple alleles and serves crucial and nonredundant roles in B-cell development and activation. B-lymphocytes play an important role in development of autoimmune diabetes in NOD mice, therefore we examined the patterns of expression of CD72 and its alloantigens on splenic lymphocytes from 4-week-old NOD/LtJ mice. Comparisons of CD72 expression were made between NOD mice and three non-diabetic strains, NON/LtJ mice, C57BL/6J and AKR/J. Use of allele-specific monoclonal antibodies revealed that the previously uncharacterized NON strain expresses either the a or d allele, whereas NOD and AKR mice were confirmed to express the rare c allele. Flow cytometric analysis revealed differential expression between the strains. Whereas NON, C57BL/6 and AKR mice expressed CD72 on 98, 94 and 92% of their B-lymphocytes (CD19+ cells), the NOD mice only expressed this regulatory marker on 78% of their B-lymphocytes. Furthermore, CD72 expression levels on CD72 positive cells were lower in NOD mice than in other three non-diabetic strains. The presence of the CD72c allele, as well as its low level of expression in NOD mice at 4 weeks of age, may be associated with B-lymphocyte hyper-responsiveness and resistance to activation-induced cell death.

Original languageEnglish (US)
Pages (from-to)115-120
Number of pages6
JournalImmunology Letters
Volume103
Issue number2
DOIs
StatePublished - Mar 15 2006

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Inbred NOD Mouse
Inbred C57BL Mouse
B-Lymphocytes
Alleles
Inbred AKR Mouse
Isoantigens
Type 1 Diabetes Mellitus
Cell Death
Monoclonal Antibodies
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Strain differences in allele and expression levels of CD72 on B-lymphocytes from NOD, AKR, NON and C57BL/6 mice. / Wu, Jian; Marler, Jacob; Lenchik, Nataliya I.; Gerling, Ivan.

In: Immunology Letters, Vol. 103, No. 2, 15.03.2006, p. 115-120.

Research output: Contribution to journalArticle

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